摘要
为探究污水处理用光合细菌PSB-B的最适生长条件,本文在单因素实验的基础上,以温度、pH和盐浓度为实验因素,根据旋转中心组合设计原理,经Design-Expert软件分析数据,采用三因素五水平的响应面法进行优化。确定光合细菌PSB-B的最适生长条件为:温度28℃,pH6.80,盐浓度0.25g/100mL。在此条件下,OD值为1.446。说明响应面法优化光合细菌PSB-B的生长条件是可行的。
The most suitable growth conditions of photosynthetic bacteria PSB-B were studied for replication.On the basis of single factor experiment,the method of response surface analysis with 3 factors and 5 levels including temperature,pH and NaCI concentration were adopted through design-expert software with central composite rotatable design analyzing data. The most suitable growth conditions of photosynthetic bacteria PSB-B were determined as following, temperature 28℃, pH value 6.80 and NaCI concentration 0.25g/100mL.Under these conditions,OD of strain PSB-B was 1.446.The results showed that the experimental value was comparable to model prediction and the method was practical.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第15期230-233,共4页
Science and Technology of Food Industry
基金
中国博士后基金(2011M501444)
山西省优秀人才支撑项目
山西省大学生创新性实验项目(105053-2011141)
关键词
光合细菌
响应面优化
温度
PH
盐浓度
photosynthetic bacteria
response surface optimization
temperature
pH
NaCI concentration
