二次正交旋转组合设计优化黑曲霉菌株F1降解酒石酸的条件研究

Research of degradation of tartaric acid by Aspergillus niger F1 by using the quadratic orthogonal rotational combination design

为提高酒石酸降解率,以黑曲霉降酸菌株F1为研究对象,采用四元二次正交旋转组合设计研究黑曲霉菌株F1培养温度、孢子悬浮液接种量、培养基装液量及酒石酸浓度4个因素对酒石酸降解率的影响,用DesignExpert7.1软件分析得出4个因素的回归方程。结果表明,黑曲霉降酸菌株F1对酒石酸的最佳降解条件为:培养温度34.7℃、接种量4.3%、装液量163mL、酒石酸浓度9.3g/L。在此条件下,其降解率可达到98.3%。

A quadratic orthogonal rotation combination design was used to explore the effects of incubation temperature,inoculation amount of spore suspension, medium volume, tartaric acid concentration of Aspergillus niger F1 on degradation rate of tartaric acid. A regression equation was established using Design Expert 7.1 software.The optimum tartaric acid-degrading conditions were incubation temperature of 34.7℃, inoculation amount of 4.3% ,medium volume 163mL,tartaric acid concentration 9.3g/L.Under such conditions, its degradation rate was 98.3 %.