几种氨基多糖和明胶对人脐静脉内皮细胞增殖和形态的影响

The effect of several glycosaminoglycans and gelatin on the proliferation and morphology of human umbilical vein endothelial cells

目的探讨羧甲基壳聚糖、羟乙基壳聚糖、硫酸软骨素、肝素和明胶对人脐静脉内皮细胞(HUVEC)的增殖和形态的影响。方法在体外进行HUVEC细胞培养,通过MTT法评价上述氨基多糖和明胶在不同浓度下对HUVEC的增殖作用及形态影响。结果上述氨基多糖和明胶在0～2 000mg·L-1浓度内均不会抑制HUVEC的增殖,部分浓度下会促进细胞生长。羧甲基壳聚糖、羟乙基壳聚糖、肝素以及明胶的最佳浓度均为500mg·L-1,而硫酸软骨素的最佳浓度为1 000mg·L-1。各实验组的细胞形态与对照组相比无明显差异。结论羧甲基壳聚糖、羟乙基壳聚糖、硫酸软骨素、肝素和明胶与HUVEC均有良好的相容性,在部分浓度时会促进HUVEC的增殖。

Objective To investigate the effect of carboxymethyl chitosan, hydroxyethyl chitosan, chon- droitin sulfate, heparin and gelatin on the proliferation and morphology of human umbilical vein endo-thelial cells（HUVEC）. Methods Cultured HUVEC in vitro, and then evaluated the effect of the above glycosaminoglycans and gelatin at different concentrations on the proliferation and morphology of HU-VEC by MTT method. Results The above glycosaminoglycans and gelatin didn＇t inhibit the prolifera-tion of HUVEC at the concentration range of 0-2 000 mg·L^-1 , and promoted the growth of HUVEC at certain concentrations within that range. The optimal concentration of carboxymethyl chitosan was 500 mg·L^-1 , as well as hydroxyethyl chitosan, heparin and gelatin. The optimal concentration of chon-droitin sulfate was 1 000 mg·L^-1. There was no obvious difference in cell morphology between each ex-perimental group and the control group. Conclusion Carboxymethyl chitosan, hydroxyethyl chitosan, chondroitin sulfate, heparin and gelatin had good cytocompatibility with HUVEC, and promoted the proliferation of HUVEC at certain concentrations.