蓝莓枝枯病病原菌鉴定

Identification of the pathogen causing twigs and stem dieback in blueberry

蓝莓（Vacciniumspp．），是一类属于杜鹃花科（Ericaceae）越橘属（Vaccinium）的多年生小浆果类果树，广泛地应用于医药、保健、化妆品和环境保护等各方面，联合国粮农组织将其果实列为人类世界五大健康食品之一，也是我国南方酸性土丘陵地区值得发展的经济作物。全国已超过10个省份开始大面积的蓝莓商业化栽培，预计未来10年将超过10万hm2。

The identification of the pathogens isolated from the stem dieback and twigs blight of Highbush Blueberries （Vaccinium corymbosum L. ） were carried out. 29 diseased samples were observed in Kunming and Lijiang Yunnan Province （south-western China）. 29 isolates of possible pathogenic fungi were isolated from stem and twigs of infected blueberry plants. Bases on the pathogenicity experiment, the results showed that LI- JIANG22 was the pathogens of Highbush Blueberries dieback disease. The morphological identification showed that LIJIANG22 was identified as Neofusicoccum parvum. Identity was confirmed by the analysis of rDNA inter- nal transcribed spacer region （ITS1-5.8S-ITS2） and the translation elongation factor 1-alpha （EFl-α）. BLAST searches at GenBank showed a high identity with N. parvum reference sequences （ITS： 〉99% ; EFI-α： 100% -99% ）. Representative sequences of isolates from both regions were deposited in GenBank （ITS： Accession No. JX096632, isolate LIJING22 ; JX096634, isolate LIJIANG23 ; EFl-α： Accession No. JX096636, JX096637）. Morphological and molecular identification confirmed this species as N. parvum. N. parvum was first reported as the pathogen of Highbush Blueberries dieback disease in China.