匙羹藤提取物干预KKAy小鼠脂肪组织胰岛素抵抗的实验研究

Experimental study on effect of Gymnema sylvestre on insulin resistance in adipose tissue of KKAy mice

目的:探讨匙羹藤(GS)改善KKAy小鼠脂肪组织胰岛素抵抗(IR)的作用机制。方法:KKAy小鼠分为模型组(DM)和GS组,C57BL/6J小鼠为正常组(NC),给药8周后测定血清胰岛素(Fins)、胰岛素敏感指数(ISI)、过氧化物酶增殖体受体-γ(PPAR-γ)、PI3κ-p85、Cb1衔接蛋白(CAP)、葡萄糖转运蛋白4(GluT-4)mRNA水平。结果:与DM比较,FPG、Fins降低,ISI升高,PPAR-γmRNA、PI3κ-p85mRNA、CAPmRNA、GluT-4 mRNA表达量升高。结论:GS可上调脂肪组织PPAR-γ、PI3κ-p85、CAP、GluT-4mRNA的表达量,改善IR。

To study the effects and mechanisms of Gymnema sylvestre on improvement of insulin resistance in adipose tissue of KKAy mice. Methods： 18 KKAy mice were divided randomly according to body weight level into Models （DM） Gymnema sylvestre group （GS） and 9 normal C57BL/6J mice as normal control group（NC）. Fasting plasma glucose（ FPG ） of all mice were tested at every weekend, and at the end of the experiment all animals were tested FPG, Fasting insulin level（Fins） for evaluating Insulin Sensitivity Index （ISI） . The expressions of PPAR-γmRNA,PI3 κ-p85mRNA,CAPmRNA and GluT-4mRNA were determined by Real-time PCR. Results： Compared with DM group, Gymnema sylvestre down- regulated the levels of FPG, Fins, up-regulated ISI and the expressions of PPAR-γ/mRNA, PI3 κ-p85mRNA, CAPmRNA and GluT-4mRNA in adipose tissue. Conclusion： Gymnema sylvestre improved insulin resistance of KKAy mice by increasing the expressions of PPAR-γmRNA,PI3 κ-p85mRNA,CAPmRNA and GluT-4mRNA in adipose tissue.