摘要
通过酶切、连接、转化等技术 ,将大麦黄矮病毒抗性基因复制酶基因ORF2插入Ti质粒pGA4 82的T DNA区 ,从而提供了新的目的基因转化小麦的农杆菌 /质粒系统 .
A new Agrobacterium tumefaciens/ Ti plasmid system is supplied with BYDV(Barley yellow dwarf virus) resistant gene for wheat transformation.By using gene clone methods,such as restriction endonuclease digest,ligation,bacterium transformation,and so on,plasmid pPPI12 with BYDV gene was inserted into T DNA region of Ti plasmid pGA482.
出处
《山东大学学报（自然科学版）》
CSCD
2000年第3期322-326,共5页
Journal of Shandong University(Natural Science Edition)
基金
国家863生物技术资助项目!(DH -0 1 -0 2 )
