獐毛愈伤组织耐盐性研究及耐盐变异体的筛选

STUDY ON SALT-TOLERANCE OF CALLI OF AELUROPUS LITTORALIS VAR.SINESIS AND SELECTION FOR HIGH SALT-TOLERANT VARIANT

獐毛 (Aeluropuslittoralisvar.sinensis)匍匐茎茎尖来源的原始型愈伤组织 ,测定其耐盐范围为 0 .3 %～ 1 .5% .盐胁迫下 ,脯氨酸及K+、Na+、Ca2 +离子在渗透调节中起重要作用 .用直接筛选的方法 ,将原始型愈伤组织放在含 2 %NaCl的选择培养基上连续筛选 ,获得耐盐性进一步提高的愈伤组织耐盐变异体 ,在选择培养基上 ,变异型愈伤组织鲜重相对生长率显著大于原始型愈伤组织 ,在变异型愈伤组织耐盐性提高中 ,脯氨酸及无机离子K+、Na+、Ca2 +起重要作用 .耐 2 %NaCl的变异型愈伤组织在无NaCl培养基中培养 1 1代后 ,仍能耐受 2 %NaCl,愈伤组织的耐盐性没有退化 ,说明筛选得到的耐盐变异体不是生理适应性的而是由遗传变异而来的 .

Calli of Aeluropus littoralis var. sinesis were initiated from the apex of creeping stem.It′s maximum salt tolerance measured was 1.5% NaCl.Proine,K + and Na + play an important role in the osmotic regulation of such calli.After repeated subculture on medium cntaining 2% NaCl,a variant type of calli with tolearance up to 2% NaCl was selected.On the medium for selection,the relative growth rates of fresh weight of the variant type calli was remarkably greater that that of the original type,and proline,K + and Na + were involved in the promotion of salt tolerance of the variant calli.After 11 terms of NaCl free subculture,the vatiant type calli could still tolerate up to 2% NaCl,indicting that the selected high salt resistant calli were genetic variant and not a physiological adapted type.