开通玄府法对胶原酶诱导的脑出血大鼠神经细胞水肿的作用评价

Evaluation of Xuanfu-Opening Method on Edema Post ICH Induced by Collagenase

目的:观察以开通玄府为立法的利开灵对胶原酶诱导的脑出血大鼠神经细胞水肿的干预作用。方法:采用Rosenberg法[1]改良后建立大鼠脑出血模型。大鼠分为利开灵组、清开灵组、安宫牛黄组,动物术后分别给予利开灵浓煎剂、清开灵口服液、安宫牛黄丸混悬液灌胃,每天1次,共3 d。术后72 h取材,置于4%多聚甲醛固定液中外固定2周。随后将脑组织修块、脱水、石蜡包埋。选取大鼠的神经功能缺损体征、脑含水量、大鼠皮层区病理学改变为观察指标。结果:模型组大鼠神经功能缺损最重,利开灵组、清开灵组及安宫牛黄组大鼠神经功能缺损较模型组明显减轻。利开灵组大鼠血肿周围可见红细胞侵润,间质及神经元肿胀明显减轻;利开灵组大鼠皮层未见明显的血管间隙加宽,间质水肿不明显。结论:研究发现利开灵在改善动物偏瘫及抓握能力等行为及脑系数、脑组织含水量、及大鼠脑组织形态学方面,与模型组大鼠比较血肿周围神经细胞肿胀有所减轻,神经细胞脱失数量也较少,本实验在细胞层面证明了基于开通玄府法的干预手段与传统的安宫牛黄丸、清开灵等具有公认的临床疗效药物相比,其减轻脑水肿及细胞保护作用明显。

Objective： To observe the effect of protecting neuro post ICH of Likailing Decoction based on the theory of opening Xuanfu of the collagenase-induced intracerebral hemorrhage rats.Methods： Improved Rosenberg was used to establishe rat model of intracerebral hemorrhage.The rats were divided into Qingkailing group Angong Niuhuang group and Likailing group,each decoction was adminstrated after surgery,once a day for 3 days.Brain was taken out 72h after surgery,placed in 4% paraformaldehyde fixative for 2 weeks,repaired,dehydrated,and embedded in paraffin subsequently.Nerve function impairment,brain edema,and rats cortex pathology were observed.Result： The neurological impairment was the worst in model rats,in Likailing group and Qingkailing Angong Niuhuang group it was mild.Hematoma around the red blood cell area,interstitial and neuronal swelling as well as the vascular space widening and interstitial edema reduced in Likailing group significantly.Conclusion： The study found that animal behaviors such as grip were improved and brain capacity factor,brain water content and rats brain tissue morphology were less impaired in Likailing group,compared with the model group rats.And swelling around the nerve cells as well as the demyelination in nerve cell were reduced.The experiment proved at the cellular level,based on method of opening Xuanfu is effective to alleviate brain edema and protect cells.