清热解毒法基于Bcl-2/Bax等凋亡信号通路调控结肠癌的研究

Research on Regulation Effect of Detoxicated Three-Root Mixture Therapy on Apoptotic Signals Pathways Based on Bcl-2/Bax in Colon Cancer Treatment

目的:观察含清热解毒中药小鼠血清对结肠癌CT-26细胞蛋白Bcl-2、Bax、P53、Caspase-3表达的影响。方法:将BALB/c小鼠随机分为用药A组、用药B组、用药C组和模型D组。A、B、C组分别连续使用解毒三根汤灌胃5、7、10 d后,与D组一起通过眼球取血获得含药血清,并作用于结肠癌CT-26细胞,MTT法测细胞24h、48h、72h的抑制率,与未经鼠血清刺激的正常CT-26细胞分别裂解蛋白后,Western blot法检测Bcl-2、Bax、P53、Caspase-3蛋白的表达量。结果:MTT法结果显示解毒三根汤灌胃10天组,即C组对细胞的抑制率最高,该组Bax、P53、Caspase-3蛋白的表达较正常结肠癌细胞(E组)升高,Bcl-2蛋白表达较正常结肠癌细胞降低,且有统计学意义(P<0.05)。结论:解毒三根汤抑制结肠癌CT-26细胞的生长可能与抑制Bcl-2的表达,促进Bax、P53、Caspase-3的表达量有关。

Objective： To investigate the clinical effectiveness of mice serum with Detoxicated Three-Root Mixture for the expression of colon cancer CT-26＇s cell protein Bcl-2,Bax,P53,Caspase-3.And to explore the probable mechanisms of tumor cell apoptosis induced by Sangen Decoction.Methods： BALB/c mice were randomly divided into four groups：medicine group A,B and C,Model group D.Mice in group A,B and C were then continuously fed with medicine for 5,7 and 10 days.Blood of mice was taken through eyeball along with group D and then acted on CT-26.Inhibitory rate of CT-26 was observed by MTT method at 24h,48h and 72h.At end of treatment,the expressions of Bcl-2,Bax,P53 and Caspase-3 were observed by western blot method.Results： The results of MTT showed that the group of 10 days made the highest inhibition rates.In this group the protein expressions of Bax,P53 and Caspase-3 were increased compared to those of normal colon cells（group E）,and Bcl-2 was lower than that of normal cells（P〈0.05）.Conclusion： Detoxicated Three-Root Mixture can inhibit the growth of colon cancer CT-26 cell.The mechanism of it may be related to the apoptosis proteins＇ expressions of Bcl-2,Bax,P53,Caspase-3,which are adjusted by Detoxicated Three-Root Mixture.