摘要
目的:通过动物实验观察德都红花-7味散对D-GaLN急性肝损伤的保护作用及利胆、调节免疫功能的主要药效学作用。方法:以蒙药制剂"德都红花-7味散"为实验药物,设计高剂量组(0.83 g.kg-1)和低剂量组(0.5 g.kg-1)。实验各组均采用灌胃法连续给药1周。分别采用腹腔注射D-GaLN制作小鼠急性肝细胞坏死模型观察肝功能及肝脏形态学的改变,评价德都红花-7味散对化学性肝损伤的防治作用;采用小鼠腹腔巨噬细胞吞噬鸡红细胞实验,评价德都红花-7味散对巨噬细胞吞噬功能的影响;采用小鼠摘除胆囊直接称重的方法,观察德都红花-7味散对小鼠胆汁分泌的影响;采用MTT法观察徳都红花-7味散对刀豆蛋白A诱导的小鼠脾淋巴细胞增值反应的影响。结果:急性肝损伤实验结果表明,与模型组比较德都红花-7味散组和联苯双酯组均有显著降低ALT,AST作用(P<0.05),与模型组比较德都红花-7味散和联苯双酯组肝组织病理学改变明显减轻。巨噬细胞吞噬功能实验结果表明,与空白组比较德都红花-7味散组和香菇多糖组吞噬率和吞噬指数明显升高(P<0.05),与香菇多糖组比较徳都红花-7味散高剂量组吞噬率明显升高(P<0.05)。利胆实验结果表明,与空白组比较徳都红花-7味散组测定时间内胆汁分泌量升高(P<0.05)。小鼠脾淋巴细胞增值实验结果表明,与空白组比较徳都红花-7味散组OD值升高(P<0.01)。结论:德都红花-7味散对D-半乳糖胺(D-GlaN)所致小鼠急性肝损伤具有保肝降酶作用;能够明显增加小鼠胆汁分泌量;明显增强小鼠腹腔巨噬细胞吞噬功能;对ConA诱导的淋巴细胞增值有促进作用。
Objective:According to animal experiments,it is to observe the protecting function and main pharmacodynamics effects as protecting liver and gallbladder and immune regulating function of Deduhonghua-7 powder on D-GaLN-induced acute liver injury.Methods:The mice of acute liver cell necrosis model was made by intraperitoneal injection of D-GaLN to observe the liver function and morphology changes of liver,in oder to evaluate the chemical protecting function of Deduhonghua-7 powder objectively.The peritoneal macrophages phagocytosis of chicken erythrocytes was used to observe the influences of Deduhonghua-7 powder at phagocytosis function of morphology;the gallbladder removing and weighting method was used to observe the influence of Deduhonghua-7 powder at secretion of bile;MTT method was used to observe the influences of Deduhonghua-7 powder at mouse spleen,lymph cell proliferation induced by concanavalin A.Result:At acute liver injury experiment,comparing with model group,ALT and AST of mice serum in the acute liver injury group and Bifendate group were increased(P〈0.05).Comparing with model group,Deduhonghua-7 powder group and Bifendate group's tissue pathological changes were obviously decreased.At macrophage phagocytosis experiment,comparing with blank group,phagocytosis rate and phagocytosis indexes in Deduhonghua-7 powder group and Lentinan group were significantly increased(P〈0.05),comparing with Lentinan group,phagocytosis rate of Deduhonghua-7 powder group was significantly increased(P〈0.05).At choleretic experiment,comparing with blank group,the secreting of bile in determined time in the Dedhuhonghua-7 powder group was increased(P〈0.05).At mice spleen,lymph cell proliferation experiment,comparing with blank group,the OD value of Deduhonghua-7 powder group was increased(P〈0.01).Conclusion:Deduhonghua-7 powder has hepatoprotective and enzyme decreasing function on mice with acute liver injury induced by D-GaLN;it can increase mice bile secretion significantly,significantly increase the abdominal macrophage phagocytosis function and improve the proliferation of lymph cell induced by ConA.
出处
《中华中医药学刊》
CAS
2013年第7期1575-1578,共4页
Chinese Archives of Traditional Chinese Medicine
基金
内蒙古自治区卫生厅学科建设资助项目(2005061)
关键词
德都红花七味散
肝损伤
调节免疫
实验研究
Deduhonghua-7 powder
liver injury
immune regulating
experimental research