用于玻璃-聚二甲基硅氧烷灌注式细胞培养芯片的温度控制系统设计

Development of a temperature control system for glass-PDMS perfusion cell culture chips

目的设计一种用于玻璃-聚二甲基硅氧烷(PDMS)灌注式细胞培养芯片的温度控制系统,代替传统的细胞培养箱,以提高与细胞灌注培养装置的可集成性,同时便于细胞培养过程中显微镜下动态观察。方法系统采用透光性良好的铟锡氧化物(ITO)镀膜导电玻璃作为加热元件,以Pt100传感器作为测温元件,与PID微控制器构成闭环温度控制系统;玻璃-PDMS细胞培养芯片采用微丝模塑软刻工艺加工而成;利用红外热成像仪对ITO加热元件表面上温度场均匀性进行验证;有限元数值分析对ITO加热元件上细胞培养芯片空间温度场分布进行模拟。结果与结论设计的温度控制系统结构简单,控制精度达±0.2℃;加热元件表面及玻璃-PDMS灌注式细胞培养芯片上培养腔内温度场空间分布均匀(1℃内),可满足实际细胞培养条件;在芯片上进行人肺癌细胞A549灌注培养实验显示,系统为细胞培养提供合适的温度环境且适合于细胞生物学行为的动态表征。

Objective To design a temperature control system for glass-polydimethylsiloxane（PDMS） perfusion cell culture chips to replace the traditional cell culture incubator.This system has a better integration with cell infusion culture devices and facilitates continuous observation under a microscope in the process of cell culture.Methods In this system,the transparent indium tin oxide（ITO） coated conductive glass was used as a heating element,a Pt100 sensor was used as temperature sensor,and a closed loop temperature control system was constructed with a PID controller.A glass-PDMS cell culture chip was fabricated using micro-wires molding technology.The temperature field distribution on the ITO heating element surface was verified by infrared thermal imaging experiments.Space temperature field distribution in the cell culture chip on this ITO heating element was checked through numerical simulation.Results and Conclusion The designed temperature control system had a simple structure and a good accuracy of 0.2℃.A uniform space temperature field distribution could be obtained on the heating element surface and in the cell culture chip culture cavity.Human lung cancer cells A549 perfusion culture experiments show that this designed system not only provides a fine temperature environment for cell culture,but is applicable to dynamic characterization of biological behavior of cells.