参芪扶正注射液的化学成分研究(Ⅱ)

Chemical constituents of Shenqi Fuzheng Injection(Ⅱ)

目的研究参芪扶正注射液(党参、黄芪)的化学成分。方法采用硅胶、ODS、Sephadex LH-20及RP-HPLC等色谱方法分离参芪扶正注射液的化学成分,通过理化性质及波谱分析鉴定化合物的结构。结果从参芪扶正注射液原料药浸膏中分离得到15个化合物,分别鉴定为黄芪皂苷Ⅰ(1)、黄芪皂苷Ⅱ(2)、黄芪皂苷Ⅲ(3)、brachyoside B(4)、异黄芪皂苷Ⅱ(5)、黄芪甲苷(6)、6″-O-乙酰基毛蕊异黄酮7-O-β-D-吡喃葡萄糖苷(7)、异落叶松脂素(8)、红车轴草素7-O-β-D-吡喃葡萄糖苷(9)、刺果甘草苷D(10)、丁香脂苷(11)、松柏苷(12)、(3R)-7,2'-二羟基-3',4'-二甲氧基异黄烷(13)、(6aR,11aR)-3-羟基-9,10-二甲氧基紫檀烷(14)及丁香酸(15)。结论化合物1～4及7～15为从该注射液中首次分离得到,化合物8和11在党参属和(或)黄芪属植物中为首次报道。

AIM To investigate the chemical constituents of Shenqi Fuzheng Injection（Codonopsis Radix,Astragali Radix）.METHODS The concentrate of Shenqi Fuzheng Injection was separated and purified by silica gel,ODS,Sephadex LH-20 and RP-HPLC column chromatographies.The structures were identified by their physicochemical properties and spectroscopic data.RESULTS Fifteen compounds were isolated and identified as astragaloside Ⅰ（1）,astragaloside Ⅱ（2）,astragaloside Ⅲ（3）,isoastragaloside Ⅱ（4）,brachyoside B（5）,astragaloside Ⅳ（6）,calycosin 7-O-β-D-glucopyranoside-6″-O-acetate（7）,（＋）-isolariciresinol（8）,pratensein 7O-β-D-glucopyranoside（9）,licoagroside D（10）,liriodendrin（11）,coniferin（12）,（3R）-7,2＇-dihydroxyl3＇,4＇-dimethoxy-isoflavan（13）,（6aR,11aR）-3-hydroxy-9,10-dimethoxy pterocarpan（14） and syringic acid（15）.CONCLUSION Compounds 1-4 as well as 7-15 are isolated from this injection for the first time.Compounds 8 and 11 are isolated from Codonopsis and / or Astragalus for the first time.