超高效液相色谱法测定贵州产灯盏细辛中的6种成分

Determination of Six Components in Erigeron breviscapus of Guizhou by UPLC

建立了超高效液相色谱法测定灯盏细辛中的飞蓬苷(1)、绿原酸(2)、野黄芩苷(3)、3,5-O-二咖啡酰基奎宁酸(4)、灯盏甲素(5)和4,5-O-二咖啡酰基奎宁酸(6)的含量。采用BEH C18色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,多波长检测[260 nm(1),326 nm(2、4、6),335 nm(3、5)]。1～6分别在0.025～0.8、0.005～0.16、0.013～0.4、0.015～0.48、0.008～0.24和0.005～0.16 mg/ml范围内线性关系良好;平均回收率分别为97.2%、96.8%、100.1%、99.5%、99.2%和101.2%,RSD分别为2.1%、1.7%、1.9%、2.6%、2.5%和2.3%。

An UPLC method was established for the determination of erigeside（1）, chlorogenic acid（2）, secutellarin（3）, 3,5-di-O-caffeoylquinic acid（4）, secutellarin methyl ester（5）and 4,5-di-O-caffeoylquinic acid（6）in Erigeron breviscapus. A BEH C18 column was used with the mobile phase of acetonitrile-0.1% phosphoric acid by gradient elution. The detection wavelength was 260 nm （1）, 326 nm （2, 4 and 6） and 335 nm （3 and 5）. Their calibration curves were linear in the ranges of 0.025 - 0.8, 0.005 - 0.16, 0.013 - 0.4, 0.015 - 0.48, 0.008 - 0.24 and 0.005 - 0.16 mg/ml, respectively. Their average recoveries were 97.2%, 96.8%, 100.1%, 99.5%, 99.2% and 101.2%, with RSDs of 2.1%, 1.7%, 1.9%, 2.6%, 2.5% and 2.3%, respectively.