栀子炮制品的HPLC指纹图谱

HPLC Fingerprints of the Prepared Medical Materials of Fructus Gardeniae

比较研究了栀子不同炮制品的高效液相色谱指纹图谱。采用CenturySIL C18BDS柱,以1%乙酸-1%乙酸乙腈溶液为流动相,低压线性梯度洗脱,检测波长265 nm,测定栀子6种炮制品的HPLC指纹图谱。以栀子苷峰为参照物峰,确定35个共有峰。栀子不同炮制品在化学成分分布上极为相似,但各组分的含量相差较大。经加热炮制后,随炮制条件加剧,大部分组分含量趋下降,但也有个别组分含量升高。

The HPLC fingerprints of the prepared medical materials of Fructus Gardenia with different process were investigated. A CenturySIL C18 BDS column was used, with 1% acetate acid-1% acetate acid acetonitrile solution as the mobile phase by gradient elution, at the detection wavelength of 265 nm. Thirty-five co-possessing peaks were selected as the fingerprint peaks by taking geniposide peak as the reference peak. There were good similarities between the HPLC fingerprints of the prepared medical materials of Fructus Gardenia in terms of the distribution of chemical ingredients, but the quantitative similarities were not desirable. Accompanied with the intensification of the heating prepared conditions, the quantity of most ingredients showed decreased trend while a few showed increased trend.