IGFBP3基因-185C>T多态与抗阻训练后肌肉力量反应的关联性研究

Association of IGFBP3-185C>T Genetic Polymorphisms with the Muscle Strength Response to Resistance Training

目的:探讨胰岛素样生长因子结合蛋白3(insulin-like growth factor binding protein 3,IGFBP3)基因-185C>T单核苷酸多态性(single nucleotide polymorphism,SNP)与非惯用手上臂抗阻训练后肌肉力量反应的关系。方法:选择福建省某大学健康的大学生600人参加本次研究;使用标准化的调查表获得受试者的基本资料;在受试者进行非惯用手上臂抗阻训练前后,使用等速肌力测试训练系统(ISOMED2000,德国D&R公司)测定双侧肘部屈肌最大随意收缩量(maximal voluntary contraction,MVC)和双侧肘部屈肌单次重复最大肌力(one rep-etition maximum,1-RM),使用磁共振成像(MRI)1.5T系统(MAGNETOM Avanto,德国西门子公司)测量双侧肱二头肌横断面积(cross sectional area,CSA)。采用高通量实时荧光定量PCR系统(7900HT,美国ABI公司)检测IGFBP3-185C>T位点基因型。结果:IGFBP3-185C>T位点基因多态性与肌肉力量反应存在着关联;抗阻训练后,在男性和女性受试者中CC基因型个体训练手MVC值分别增加了22.5%和24.5%,同时,分别在两组受试者中,CC基因型个体训练手MVC值变化量均显著高于CT、TT基因型个体(P<0.05);在女性受试者中,CC基因型个体非训练手1-RM值和训练手CSA值分别增加了17.4%和21.1%,同时,CC基因型非训练手1-RM值和训练手CSA值均较CT+TT基因型个体显著增加(P<0.05);进一步研究发现,随着T等位基因的增加男女训练手MVC值及其该变量以及女性非训练手1-RM和训练手CSA值均逐渐降低,趋势检验发现,具有剂量效应关系(P<0.01)。结论:IGFBP3基因-185C>T位点与受试者抗阻训练后肌肉力量反应存在着关联。

Objective：To explore the associations between the insulin-like growth factor binding protein 3（IGFBP3-185CT polymorphisms and the muscle strength response to a unilateral,upper arm resistance-training program among healthy,young adults.Methods：A total of 600 healthy young adults from university in Fujian province were included in the present study.Participants＇ information and data were obtained by a standard questionnaire.Polymorphisms of IGFBP3-185CT were detected by Taqman single nucleotide polymorphism（SNP） allelic discrimination by means of an ABI7900HT.All subjects performed a training program of the nondominant（trained） arm with the dominant（untrained） arm as a comparison.Peak elbow flexor strength was measured with one repetition maximum,isometric strength with maximum voluntary contraction,and bicep cross-sectional area with MRI in the trained and untrained arms before and after training.Results：there existed a connection between IGFBP3-185CT gene polymorphism and muscle strength response at;after resistance training,in male and female subjects,the MVC values of CC genotype individual trainers respectively increased by 22.5% and 24.5%,meanwhile,in the two groups of subjects,the variation of MVC value of CC genotype individual trainers was obviously higher than that of CT and TT genotype individuals（P0.05）;in female subjects,the 1-RM value of CC genotype individual trainers and CSA value of trainers respectively increased by 17.4% and 21.1%;meanwhile,both-RM value of CC genotype individual trainers and CSA value of trainers increased compared with CT＋TT genotype individuals（P0.05）;it was found from the further research that with the increasing of T allele,the MVC value of male and female trainers and the variable as well as 1-RM of female non-trainers and CAS value of trainers all gradually reduced,it was found that there existed a dose-effect relationship（P0.01） through trend test.Conclusion：The IGFBP3 C185 allele may increase more muscle strength response to training in men and women.