快速溶剂萃取-液相色谱法测定人参药材中人参皂苷的含量

Determination of ginsenosides in Ginseng Radix et Rhizoma by ASE-HPLC

目的:建立快速溶剂萃取(ASE)-液相色谱法测定人参药材中人参皂苷Re、Rg1、Rb1、Rc、Rb2、Rd。方法:用三氯甲烷脱脂,水饱和正丁醇提取药材中人参皂苷;采用C18柱,乙腈-水为流动相进行梯度洗脱,检测波长为203 nm,柱温为35℃,进行含量测定。结果:人参皂苷化合物响应值与进样量呈良好线性关系(r>0.999),测定精密度RSD﹤2.0%。结论:本法操作简便、快速,结果准确,可用于人参药材质量控制。

Objective： To establish an accelerated solvent extraction（ASE）-HPLC method to determine ginsenosides Re,Rg1,Rb1,Rc,Rb2,Rd in Ginseng Radix et Rhizoma.Methods： Trichloromethane was used to remove the lipid ingredients and water-saturated butanol solution was adopted to extract the ginsenosides.A C18 column with mobile phase of acetonitrile aqueous solution was used,and the contents of ginsenosides were determined at a wavelength of 203 nm with the column compartment set at 35 ℃.Results： The response factor of ginsenosides regarding to the different injections was linear,which generated a satisfactory correlation coefficient（r 0.999）.The method precision showed a sound coefficient（RSD% 2.0%）.Conclusion： The method is simple,fast and accurate,which is suitable for quality control of Ginseng Radix et Rhizoma.