HPLC-FLD快速测定大豆中的多环芳烃

Rapid assay of polycyclic aromatic hydrocarbons in soybean by HPLC-FLD

建立了大豆中多环芳烃的快速检测方法。采用乙腈-四氢呋喃(1+1)提取大豆中的多环芳烃,并用高效液相色谱-荧光光谱(HPLC-FLD)法测定多环芳烃含量。方法检出限和定量限分别为0.07～0.61μg/kg和0.27～2.04μg/kg,线性范围为2～200μg/kg,回收率在81.7%～96.5%之间,相对标准偏差在2.10%～10.44%之间。

A rapid method for detecting polycyclic aromatic hydrocarbons （PAHs） in soybean was devel- oped. After the soybean was treated with acetonitrile - tetrahydrofuran （ 1 ： 1 ） , the PAHs contents were determined by HPLC - FLD. The detection limit and the limit of quantification of the method were 0.07 - 0.61 μg/kg and 0.27 -2.04 μg/kg, respectively. For the twelve PAHs, the method had a good linear range, which was 2 - 200 μg/kg. The recovery rate ranged from 81.7% to 96.5% and the relative standard deviation was in the range of 2.10% - 10.44%.