盐胁迫对菘蓝基因组DNA甲基化的影响

Effect of Salt Stress on DNA Methylation in Isatis indigotica

目的:分析盐胁迫对菘蓝基因组DNA甲基化模式的影响。方法:采用甲基化敏感扩增多态性(methyla-tion sensitive amplification polymorphism,MSAP)方法。结果:使用15对MSAP选择性引物对盐胁迫前后菘蓝基因组进行甲基化敏感多态性扩增,共得到632条清晰的带纹。共检测到菘蓝基因组44(6.9%)个CCGG位点在盐胁迫后发生了甲基化状态的改变,其中31(4.9%)个位点发生了超甲基化,13(2.0%)个位点发生了去甲基化。结论:盐胁迫能引发菘蓝基因组CCGG位点甲基化模式的改变。

Objective：To investigate the effect of salt stress on DNA methylation in lsatis indigotica. Methods：Methylation sensitive amplification polymorphism（MSAP） approach was used in this study. Results：By using 15 pairs of selective primers combinations, a total of 632 MSAP bands were obtained in Isatis indigotica genome under salt stress. In all, 44 （ 6.9%） CCGG sites displayed cytosine methylation changes under salt stress. Among these sites, 31 （4.9 % ） CCGG sites underwent hypermethylation changes and 13 （2.0%） CCGG sites underwent demethylation changes. Conclusion：This result provides evidence for DNA methylation remodeling occurring immediately after salt stress affects Isatis indigotica.