基于响应曲面设计法筛选蚯蚓蛋白烧烫伤喷雾剂处方的研究

Optimization the Formulation of Eisemia foetida Protein Burn Spray by Response Surface Methodology

目的:筛选出蚯蚓蛋白烧烫伤喷雾剂最佳处方。方法:采用五因素三水平的响应曲面设计,以蚯蚓蛋白(EFP)对NIH3T3细胞增殖率为主要指标,考察处方中不同组分之间的相互影响,优化处方。结果:蚯蚓蛋白烧烫伤喷雾剂最佳处方组成为:EFP含量0.91%,甘油含量1.42%,甘露醇含量5%,0.02 mol/L Na2HPO4和0.01 mol/L柠檬酸缓冲体系对应的pH值为7.0,尼泊金乙酯含量0.01%。结论:基于响应曲面法筛选出的蚯蚓蛋白烧烫伤喷雾剂效果良好,且方法可靠,较为简便。

Objective ：To optimize the formulation of Eisemia foetida protein （EFP）burn spray. Methods：A five-factor, three-level response surface method was employed ;The response variable was the proliferation effect of EFP on NIH3T3 cells. Results ：The optimization formulation was as follows：the proportion of EFP, glycerol and mannitol was 0. 91%, 1.42% and 5% , respectively; 0. 02 mol/L Na2 HPO4 and 0. 01 mol/L citric acid buffer system corresponding pH value was 7.0. Conclution：The response surface method is reliable, efficient and suitable for optimizing the formulation of EFP burn spray.