大孔吸附树脂对葛根中3种异黄酮类化合物的吸附分离研究

Adsorption and Isolation of Macroporous Resin for Three Isoflavonoids from Puerariae Lobatae Radix

目的:选择适合分离纯化葛根中3种成分的大孔吸附树脂。方法:以3'-羟基葛根素、葛根素及大豆苷的吸附率及解析率为评价指标,考察葛根3种异黄酮成分的吸附与解吸能力、吸附等温线、漏出曲线及洗脱曲线。结果:XDA-5树脂分离纯化3'-羟基葛根素、葛根素及大豆苷的较优工艺条件为:上样液浓度为每1 mL含葛根0.5～0.8 g,流速2 BV/h,乙醇梯度洗脱,洗脱速度1 BV/h。结论:该工艺条件所得纯化物中,3'-羟基葛根素、葛根素、大豆苷的含量分别为23.39%、52.09%和19.21%。

Objective：To study the adsorption function of macroporous resins for the separation and purification of three isoflavonoids （ 3 ＇-hydroxypuerarin, puerarin, daidzin） in Puerariae Lobatae Radix. Methods ： The contents of 3 ＇-hydroxypuerarin, puerarin, daidzin were determined by HPLC. D101, AB-8, XDA-5, HP-20 and other five resins were systematically investigated for their adsorption capability, adsorption isothermal curve, adsorption and elution properties with static and dynamic experiments. Results： Experimental resuits showed that macroporous resin XDA-5 owned optimum adsorption and elution parameters. Optimal conditions for adsorption and e- lution the three components were： per lmL solution contained Puerariae Lobatae Radix 0. 5 -0. 8 g, flow rate 2BV/h, adsorbed 3＇- hydroxypuerarin, puerarin and daidzin could be desorbed by alcohol at flow rate 1 BV/h. Conclusion：The purity of 3＇-hydroxypuerarin, puerarin and daidzin of the product obtained from Puerariae Lobatae Radix with optimum technology is 23.39%, 52. 09% and 19.21%, respectively.