1株鸿雁源新城疫强毒的分离鉴定

Isolation and Identification of High Virulent Newcastle Disease Virus Strain in Swan Goose

为了查明某大雁养殖场大雁发病原因,应用血凝和血凝抑制试验对病原进行了分离鉴定,同时对其致病性进行了测定。结果表明,该分离病毒为新城疫病毒(命名为DQBT01),该病毒能使鸡红细胞发生凝集,且这种凝集能被鸡新城疫标准阳性血清所抑制,同时其F基因检测阳性。经测定,其鸡胚平均死亡时间(MDT)、1日龄雏鸡脑内接种的致病指数(ICPI)和6周龄雏鸡静脉接种致病指数(IVPI)分别为55h、1.68、2.54,表明该新城疫病毒为强毒。本次病毒分离表明,在人工饲养条件下,野生水禽可以感染新城疫病毒并导致发生疾病,因此提示有必要对此病毒做进一步研究。

In order to identify the disease causes of swan goose, the pathogen was isolated and identi- fied by hemagglutination and hemagglutination inhibition test, and its pathogenicity was determined, in May 2012. The results showed that the isolated virus was identified to be Newcastle disease virus （NDV） based on the serological test （named DQBT01）. And the indexes of mean death time （MDT） of chicken embryo, intracerebral pathogenicity index （ICPI） in one-day-old chicken and intravenous pathogenicity index（IVPI）was 55h, 1.68, 2.54 respectivelY. The results demonstrated that the isolate was a highly pathogenic strain. Swangeese in captivity infected with NDV and so it is necessary to further research on the virus.