福建华溪蟹线粒体DNA COI和16S rRNA基因序列的遗传多样性

Genetic diversity on mitochondrial DNA COⅠ and 16S rRNA of Sinopotamon fukienense

目的探讨福建华溪蟹(Sinopotamon fukienense)的遗传多样性。方法采用PCR结合DNA测序技术,测定S.fukienense的线粒体COI和16SrRNA基因序列的组成。经比对获得639bp长度的COI基因序列和526bp的16SrRNA基因序列,以对比分析S.fukienense的遗传多样性。结果 S.fukienense基于COI基因核苷酸多样性(Pi)为0.048 4,高于其基于16SrRNA基因核苷酸多样性(Pi)为0.021 6。同时,S.fukienense基于COI基因单倍型间的平均遗传距离(P)为0.048,大于其基于16SrRNA基因单倍型间的平均遗传距离(P)0.026。结论 COI序列在分析S.fukienense遗传异变时的作用更优于16SrRNA基因序列。

In order to explore the genetic diversity of the freshwater crab S.fukienense,the base composition and sequence variation of COⅠand 16S rRNA of it were studied by the methods of PCR and DNA sequencing.We obtained 639bp nucleotide sequence of gene COI and 526bp nucleotide sequence of gene 16S rRNA.The nucleotide diversity based on gene COI（Pi= 0.0484） is higher than that based on 16S rRNA（（Pi= 0.0216）.At the same time, The average genetic distance of haplotypes based on gene COI （P=0.048） was larger than that based on 16S rRNA （P=0.026）.So we think that the COI sequences is better than the 16S rRNA sequence in the analysis of genetic mutation for S.fukienense.