补中益气汤对脾虚大鼠胃粘膜MEK/ERKmRNA表达的影响

Effect of Buzhongyiqi Decoction on the expression of gastric mucosa MER /ERK mRNA in rats with the syndrome of Spleen Deficiency(脾虚)

目的:观察脾虚大鼠胃粘膜MEK、ERKmRNA表达变化及补中益气汤对其表达的影响,并探讨相关作用机制。方法:SD大鼠随机分为设空白对照组、脾虚损伤组、补中益气汤组、非脾虚损伤组。脾虚损伤组、补中益气汤组大鼠给予100%大黄水煎剂灌胃,一天2次,连续10天。第11天开始,补中益气汤组给予补中益气汤灌胃治疗7天。第18天,除空白对照组外的另外三组均灌服消炎痛造成胃粘膜损伤,采用荧光定量PCR(SYBR Green I)检测MEKmRNA、ERKmRNA。结果:与非脾虚损伤组比较,脾虚损伤组大鼠胃粘膜MEKmRNA明显下降;与脾虚损伤组比较,补中益气汤20g/kg大鼠胃粘膜MEKmRNA表达明显上调,ERKmRNA显著升高。结论:补中益气汤可提高脾虚大鼠消炎痛攻击后胃粘膜MEKmRNA、ERKmRNA表达水平,提示该方的胃粘膜保护、降低胃粘膜易损性等作用可能与激活MEK/ERK信号转导途径有关。

Object： To observed the expression of gastric mucosa MEK,ERKmRNA in the Spleen Deficient rats and the effect of Buzhongyiqitang.Methods： The rats were randomly divided into the spleen deficiency with injury group,The modelof spleen deficiency was copied by Dahuang,and Buzhongyiqitang group,the blank control group,and the non-spleen deficiency with injury group according to their weight.The spleen deficiency with injury group and the Buzhongyiqitang group were administrated by 100% DaHuang solution,twice a day,for 10 days.Then the Buzhongyiqitang group was treated by Buzhongyiqitang for 7 days,twice a day.In addition to the control group,the other three groups were fed with indomethacin solution to cause mucosa injruy after treatment.FQ-PCR was used to assay MEK、ERKmRNA.Results： Compared with the non-spleen deficiency with injury group,the expression of gastric mucosa MEKmRNA in the spleen deficiency with injury group were decreased significantly（P 0.01）;Compared with the spleen deficiency with injury group,the expression of gastric mucosa MEKmRNA in the Buzhongyiqitang group were increased significantly（P 0.05）,the expression of gastric mucosa ERKmRNA in the Buzhongyiqitang group were increased significantly（P 0.01）.Conclusion： Buzhongyiqitang could increase the expression of TFF1、EGFR to activate the MEK/ERK pathway and promote cell migration,proliferation or differentiation,which may be one of the deeper protective mechanisms of gastric mucosa of Buzhongyiqitang.