黄连解毒汤不同提取部位及相关单体对小鼠脾淋巴细胞增殖作用研究

The effect of different fractions and components in Huanglianjiedutang on the in vitro proliferation of the mouse splenic lymphocytes

目的:研究黄连解毒汤不同提取部位及小檗碱、黄芩苷、栀子苷、芦丁对小鼠T、B淋巴细胞增殖的影响。方法:建立体外脾T、B淋巴细胞模型,运用MTT比色法分析比较黄连解毒汤的不同提取部位及其主要化学成分与丝裂原(ConA、LPS)共同作用时对小鼠脾T、B淋巴细胞增殖作用的影响。结果黄连解毒汤全方水提部位(H2O部位)、醇提总部位(EtOH部位)、二氯甲烷萃取部位(CH2Cl2部位)和正丁醇萃取部位(n-Bu部位)对未活化或LPS和ConA诱导的脾T、B淋巴细胞具有不同程度的促进或抑制作用。小檗碱、黄芩苷(10μM)能促进未活化小鼠脾淋巴细胞增殖,而栀子苷、芦丁(50μM)对未活化小鼠脾淋巴细胞的增殖作用不明显。对于脂多糖(LPS)或刀豆蛋白A(ConA)诱导的脾淋巴细胞,小檗碱、黄芩苷、栀子苷、芦丁均能抑制其增殖。结论:黄连解毒汤不同提取部位及其中主要化学成分小檗碱、黄芩苷、栀子苷、芦丁对T、B淋巴细胞均有不同程度的增殖作用。

Objective： To study the effect of the different extraction and components of Huanglianjiedutang on the proliferative activities of mouse T/B lymphocytes.Method： Different fractions and components at different concentrations with or without concanavalin A（ConA） and lipopolysaccharide（LPS） were added into mouse splenic lymphocyte in vitro.Effects of fractions and components on the proliferative activities of T/B lymphocytes were measured by MTT colorimetric method.Results： Proliferative activities of cells that treated with four fractions with or without ConA and LPS were promoted at different degrees.Un-activated proliferation activities of splenic lymphocytes was promoted by berberine and baicalin（10 μM）,and not affected significantly by geniposide and rutin（50 μM）.LPS-induced proliferation of B lymphocytes or ConA-induced proliferation of T lymphocytes was significantly inhibited by berberine,baicalin,geniposide and rutin.Conclusion： Four fractions and main components in Huanglianjiedutang can enhance or inhibit the proliferative activities of mouse splenic lymphocytes in different degrees,and possess different immunoregulatory activity.