咖啡酸对化疗后骨髓抑制合并肺组织急性损伤小鼠毛细血管通透性及免疫功能的影响

Effects of caffeic acid on capillary permeability and immune function in myelosuppressed mice after chemotherapy combined with acute lung tissue damage

目的:观察咖啡酸对化疗后骨髓抑制合并细菌脂多糖诱发肺组织急性损伤小鼠毛细血管通透性及免疫功能的影响。方法:采用阿糖胞苷制备骨髓抑制小鼠模型,同时灌胃给予低(50mg/kg)、中(100mg/kg)、高(150mg/kg)不同剂量咖啡酸,给药第8～10天,给予各模型组小鼠腹腔注射细菌脂多糖,造成小鼠肺组织急性损伤和炎症。结果:疗程结束次日即第13天,阳性对照组及高剂量组体重已恢复正常,且体重明显大于模型阴性对照组;给药第9天咖啡酸高剂量组白细胞数已恢复正常,明显高于模型阴性对照组,其余各模型组仍未恢复正常;第13天咖啡酸高剂量组胸腺指数明显高于模型阴性对照组,已恢复正常;中、高剂量咖啡酸均能够明显降低合并肺组织急性炎症模型小鼠肺组织毛细血管通透性。结论:咖啡酸灌胃给药能够升高化疗后骨髓抑制小鼠体重、白细胞数、胸腺指数,降低炎症组织毛细血管通透性。

Objective： To investigate the effects of different doses of caffeic acid（CA） on capillary permeability and immune function in myelosuppressed mice after chemotherapy combined with acute lung tissue damage.Methods： Myelosuppressed mice were prepared by chemotherapy drug Cytosine arabinoside（Ara-C）,and treated with low-dose CA（50 mg/kg）,intermediate-dose CA（100 mg/kg）,and high-dose CA（150 mg/kg） by gavage meanwhile.All myelosuppressed groups were injected intraperitoneally with lipopolysaccharides on day 8~10 to cause acute lung tissue damage and inflammation.Results： On the next day after regime completion,the body weight of positive control and high-dose CA groups recovered normal,and were significantly higher than that of negative control.The white blood cell counts of high-dose CA group recovered normal on day 9 and were significantly higher than that of negative control,but the other myelosuppressed groups did not recover normal.The thymus index of high-dose CA group was significantly higher than that of negative control and recovered normal on day 13.The capillary permeability of intermediate and high dose CA groups was significantly decreased in myelosuppressed mice combined with acute lung tissue damage,compared with negative control.Conclusion： Treatment with high-dose of CA could increased the body weight,white blood cells,thymus index in myelosuppressed mice after chemotherapy and decreased the capillary permeability of inflammation tissue.