厚朴酚与和厚朴酚对大鼠回肠短路电流的影响

Effects of magnolol and honokiol on short-circuit current of rat ileum

目的:研究厚朴酚与和厚朴酚对大鼠回肠短路电流的影响。方法:应用体外Ussing chamber系统评价50μmol/L、100μmol/L、200μmol/L厚朴酚或和厚朴酚对回肠短路电流的作用。结果:厚朴酚与和厚朴酚均能明显抑制大鼠回肠的短路电流,且短路电流呈浓度依赖性降低,同时,其对短路电流的抑制效应显著高于钙通道阻滞剂维拉帕米;不同浓度的厚朴酚与和厚朴酚预处理可以显著降低10μmol/l乙酰胆碱激活的短路电流的增加。结论:厚朴酚与和厚朴酚可抑制大鼠回肠的短路电流,其作用机制可能是作为钙离子拮抗剂,通过调控肠道钙离子通道功能来抑制钙离子或其他离子的转运。

Objective： To study the effects magnolol and honokiol on the electrophysiological parameters of rat ileum.Methods： Ussing chamber system were used to evaluate the effects of 50 μmol/L,100 μmol/L,and 200 μmol/L magnolol or honokiol on ileal short-circuit current.Results： Magnolol and honokiol significantly inhibited the short-circuit current of the rat ileum,and the short-circuit current showed a magnolol or honokiol concentration-dependent reduction.The inhibitory effect of magnolol and honokiol on the short-circuit current was significantly higher（P0.01） than that of the calcium channel blocker-verapamil.In addition,pretreatment in Ussing chamber with magnolol or honkiol significantly reduced（P 0.01） the increase of short-circuit current induced by 10 μmol/L acetylcholine.Conclusion： The short-circuit current of magnolol and honokiol inhibited the short-circuit current of rat ileum and their mechanism of action may be as a calcium antagonist to inhibit the transport of calcium or other ions through intestinal calcium channel regulation.