丹红注射液联合骨髓间充质干细胞移植治疗大鼠脑梗死

Danhong injection plus bone marrow mesenchymal stem cell transplantation for treatment of cerebral infarction in rats

背景:单纯骨髓间充质干细胞移植修复受损脑组织的作用并不十分理想。目的:观察丹红注射液联合骨髓间充质干细胞移植治疗大鼠脑梗死的效果。方法:用线栓法制备大鼠大脑中动脉阻塞模型,随机分为3组,模型组尾静脉注射PBS、丹红注射液组尾静脉注射2mL/kg丹红注射液、联合治疗组联合注射2mL/kg丹红注射液+2.0×109L-1的骨髓间充质干细胞悬液,连续5d,1次/d。结果与结论:在骨髓间充质干细胞移植后2周,联合治疗组神经功能评分明显优于模型组及丹红注射液组(P<0.05);移植后3周联合治疗组大鼠脑梗死体积明显小于模型组和丹红注射液组(P<0.05);病理组织学观察也可见联合治疗组的组织损伤减轻程度大于丹红注射液组和模型组。结果可见丹红注射液联合骨髓间充质干细胞移植治疗大鼠脑梗死疗效显著,可以对脑细胞起到保护作用。

BACKGROUND： Simple bone marrow mesenchymal stem cell transplantation in repair of damaged brain tissues does not exhibit ideal effect. OBJECTIVE： To investigate the effect of Danhong injection combined with bone marrow mesenchymal stem cell transplantation on the treatment of cerebral infarction in rats. METHODS： The models of middle cerebral artery occlusion were established by suture method and randomly divided into three groups. Model group received tail vein injection of PBS. Danhong injection group received tail vein injection of 2 mL/kg Danhong injection. Combination group received injection of2 mL/kg Danhong injection ＋ 2.0×10^9/L bone marrow mesenchymal stem cell suspension, for 5 consecutive days, once a day. RESULTS AND CONCLUSION： At 2 weeks following bone marrow mesenchymal stem cell transplantation, the neurological function scores in the combination group were significantly better than those in the model group and Danhong injection group （P 〈 0.05）. Cerebral infarct volume in the combination group was significantly less than that in the model and Danhong injection groups at 3 weeks following transplantation （P 〈 0.05）. Histopathological observation showed that the reduced degree of tissue injury was larger in the combination group than the Danhong injection and model groups. Results indicated that Danhong injection combined with bone marrow mesenchymal stem cell transplantation on the treatment of rats with cerebral infarction showed remarkable effects and protected brain cells.