低剂量饮酒抑制大鼠肝组织DNA中8-oxo-dGsn水平

Inhibition of 8-oxo-dGsn in Livers of Rats after Mild Alcohol Consumption

目的检测低剂量饮酒大鼠肝组织DNA中氧化标志物8-羟基脱氧鸟苷(8-oxo-dGsn)水平,评价低剂量饮酒对肝脏组织氧化应激的影响。方法 4月龄的健康雄性SD大鼠48只,随机分为对照组和饮酒组,饮酒组每天灌胃56度二锅头酒每天每公斤低于2g(<2g/kg),对照组每天灌胃同等体积的生理盐水。在灌胃4、8、12周时,用放射性核素稀释高效液相-串联质谱(ID-LC-MS/MS)方法检测大鼠肝组织DNA中氧化标志物8-羟基脱氧鸟苷(8-oxo-dGsn)含量;用分光光度法测定抗超氧阴离子(O2-)能力和谷胱甘肽过氧化物酶(GSH-PX)活力。结果低剂量饮酒4、8、12周后的肝组织DNA中8-oxo-dGsn含量均较同时间点对照组低,差异有统计学意义(P<0.05);低剂量饮酒4、8、12周后的肝组织中抗O2-能力与GSH-PX活性均增强,与对照组相比差异有统计学意义(P<0.05)。结论低剂量饮酒可抑制大鼠肝组织DNA中的氧化水平,可能与肝脏中抗氧化酶活性的增高有关。

Objective To study the level of 8 - dihydro - 2＇ - deoxyguanosine （8 - oxo - dGsn） , a biomarker of DNA oxidation, in the livers of rats after mild alcohol consumption. Methods Totally 48 male Sprague - Dawley （SD） rats at four months of age were allocated randomly in to two groups： the normal control group and the alcohol consumption group. The rats in the alcohol group were given 56° alcohol by intragastric administration in a total dose of 2g/（ kg · d） while the the rats in the control group were given the same volume of physiological saline （0.9% , NaC1）. At the end of 4, 8, 12 weeks of treatment, we determined the level of 8 -oxo- dGsn, as a bio- marker of DNA oxidation, in livers of rats by ID - LC - MS/MS, and meanwhile, detected the ability of agonistie - superoxide anion and the active of glutathion peroxidase （ GSH - PX） by spectrophotometric method. Results The level of 8 - oxo - dGsn in the liver tissues of alcohol group was lower than that of the control group in 4, 8 and 12weeks （P 〈 0.05 ）. At the same time, the ability of agonistic - superoxide anion（ O2- ） and the active of glutathion peroxidase （ GSH - PX） in the liver tissues of alcohol group were higher than that of control group （P 〈 0. 05 ）. Conclusion These results suggest that a low dosage of alcohol consumption for rats would suppress the DNA oxidation in livers, which may be related to the active of the antioxidative enzyme raised up after drinking slightly.