稳定表达DLC-1的淋巴瘤细胞株模型的建立

Establishment of A Lymphoma Cell Line with Stable Expression of DLC-1 by Gene Transfection

目的建立稳定表达肝癌缺失基因-1(DLC-1)的淋巴瘤细胞株。方法将DLC-1真核表达质粒转染人淋巴瘤细胞株Raji,G418筛选,对获得的Raji细胞株进行Western blot鉴定。结果酶切和测序鉴定DLC-1的cDNA片段正确插入pcDNA3.1质粒,Western blot鉴定转染后Raji细胞株DLC-1高表达。结论成功建立了稳定高表达DLC-1的淋巴瘤细胞株。

Objective To establish a human lymphoma cell line that stably expresses deleted in human liver cancer - 1 gene（ DLC - 1 ）. Methods The reeonstructed plasmid,pcDNA3.1 - DLC - 1 ,was transfected to the human lymphoma cell line of Raji. Then the transfected Raji cells were selected by G418. The stable overexpression of DLC - 1 was identified by Western blot assay. Results The recombined plasmid, peDNA3.1 - DLC - 1, was confirmed by restriction endonuclease examination and sequencing. Western - blot showed that DLC - 1 protein expression was increased in the transfected Raji cells. Conclusion A human lymphoma cell line with stable expres- sion of DLC - 1 is established.