肝内胆管结石与ApoAⅠ-CⅢ-AⅣ基因PstⅠ位点多态性的关系研究

Relationship between the Apolipoprotein AⅠ、CⅢ and AⅣ Gene Cluster Polymorphism at PstⅠSite and the Happening of Hepatolithiasis

目的探讨载脂蛋白AⅠ-CⅢ-AⅣ(apolipoprotein AⅠ-CⅢ-AⅣ,Apo AⅠ-CⅢ-AⅣ)基因PstⅠ位点多态性与肝内胆管结石易感性(the happening of hepatolithiasis)的关系。方法应用PCR和限制性内切酶技术对64例肝内胆管结石患者和120例正常人Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点进行限制性片段长度多态性(restriction fragment length polymorphisms,RFLP)分析。结果肝内胆管结石组中A1A1、A1A2、A2A2基因型频率分别为0、4.7%、95.3%,正常对照组中A1A1、A1A2、A2A2基因型频率分别为0、10.0%、90.0%。肝内胆管结石组和正常对照组各基因型频率的比较(χ2=1.599,P=0.206)和等位基因频率的比较(χ2=1.531,P=0.216)差异均无统计学意义。结论 Apo AⅠ-CⅢ-AⅣ基因PstⅠ位点多态性可能不是肝内胆管结石的危险因素。

Objective To study the relationship between Apolipoprotein AⅠ,CⅢ ,AⅣ gene cluster polymorphism at Pst Ⅰ and the happening of hepatolithiasis. Methods Totally 64 hepatolithiasis patients and 120 healthy controls in Zhejiang Province were studied. Restriction fragment length polymorphisms（RFLP） at PstI,SstI sites of Apo AⅠ -CⅢ -AⅣ gene cluster were studied using polymerase chain reaction （PCR）. Results The genotype frequency of A1A1 ,A1 A2, A2A2 in the hepatolithiasis group and control group was sepa- rately 0,4.7% ,95.3% and 0,10.0% ,90.0%. The genotype frequency and gene frequency both were of no difference between hepatolith- iasis group and control group （P 〉 0.05）. Conclusion The data showed that PstI RFLP of ApoAⅠ - CⅢ -AⅣ gene cluster is not asso- ciated with hepatolithiasis in Chinese patients.