摘要
目的观察乌梅丸对实验性结肠炎大鼠脾脏组织Delta-阿片受体(DOR)的mRNA和蛋白表达的影响,旨在研究乌梅丸治疗结肠炎的新机制。方法 56只SD大鼠随机分成空白对照组、结肠炎模型组、美沙拉嗪组、乌梅丸组(每组14只)。除空白对照组外,其他3组均应用2,4,6-三硝基苯磺酸灌肠。模型建成2天后,空白对照组和结肠炎模型组分别以蒸馏水每只3ml灌胃,美莎拉嗪组用美莎拉嗪混悬液(浓度50g/L)、乌梅丸组用乌梅丸液(浓度0.515g/L)以每只3ml灌胃,连续给药15天后取脾脏组织,应用real time-PCR检测DOR的mRNA的表达,应用Western blot技术检测DOR蛋白的表达。结果与空白对照组相比,结肠炎模型组DOR的mRNA和蛋白表达显著升高(P<0.05);与结肠炎模型组相比,乌梅丸组、美沙拉嗪组DOR的mRNA和蛋白表达显著下降(P<0.05),但乌梅丸组和美沙拉嗪组DOR的mRNA和蛋白表达均无显著差异(P>0.05)。结论乌梅丸可能通过下调脾脏组织DOR的mRNA和蛋白表达,发挥治疗结肠炎的作用。
Objective To investigate the effects of Wumeiwan on the expression of delta - opioid receptor （DOR） in the spleen tis- sue of rat with experimental colitis. Methods Fidty six SD rats were randomly devided into control group,colitis model group,mesalazine group and WuMeiWan group （ 14 rats in each group）. Apart from control group, the rats in other three groups were induced in experimental colitis by 2,4,6 - trinitrobenzenesulfonic acid. And 2 days later,rats in control group and colitis model group were administered intragas- trically with normal saline at a dose of 3 ml,while rats in the mesalazine group and Wumeiwan group were intragastrically given mesalazine （50g/L） and Wumeiwan （0. 515g/L） at a dose of 3ml,respectively. All rats were treated for 15days. Spleen tissue samples were taken to detect the expression of mRNA of DOR by quantitative polymerase chain reaction （ RT - PCR） and their proteins by Western blot. Results The expression of the mRNA and proteins of DOR were significantly higher in the model group than those in the control group （P 〈 0. 05 ）. Treatment with either Wumeiwan or mesalazine significantly decreased the expression of the mRNA and proteins of DOR （ P 〈 0.05 ）. And there was no significant difference in those between the Wumeiwan group and mesalazine group （ P 〉 0.05 ）. Conclusion Wumeiwan significantly decreased the expression of the mRNA and proteins of DOR in the spleen tissue of rat with Experimental colitis
出处
《医学研究杂志》
2013年第7期77-80,共4页
Journal of Medical Research
基金
湖北省自然科学基金资助项目(2011CDB386)
