EML4-ALK融合基因与EGFR基因突变在肺腺癌中的表达

Expression of EML4-ALK and EGFR Mutation in Lung Adenocarcinoma

目的探讨肺腺癌EML4-ALK融合基因与EGFR基因突变的发生率及临床病理特征。方法采用FISH和ARMS方法对安徽省铜陵市人民医院2008～2011年手术切除和经皮肺穿刺活检病理确诊的肺腺癌60例石蜡包埋组织分别进行EML4-ALK融合基因和EGFR基因突变检测,并分析与临床病理特征的相关性。结果肺腺癌病理组织中EML4-ALK融合基因阳性表达率为8.33%,EGFR基因突变率61.67%,女性患者突变率76.92%(20/26)较男性患者突变率50.0%(17/34)高,P=0.034;吸烟<400年支突变率74.29%(26/35)较吸烟≥400支突变率44.0%(11/25)高,P=0.017;≥60岁患者突变率42.86%(12/28)较<60岁患者突变率78.13%(25/32)低,P=0.005,差异均有统计学意义。EML4-ALK和EGFR在肺腺癌患者中表达的相关性分析,显示二者之间呈负相关(R=-0.134,P=0.308),差异无统计学意义。结论 EML4-ALK在肺腺癌中低表达,EGFR基因突变高表达,两者呈负相关。

Objective To investigate the relation between EML4 - ALK fusion gene and EGFR gene positive expression in clinical and pathological characteristics. Methods The study applied FISH and the ARMS technology,with detection of the EML,4 - ALK and EGFR gene in 60 patients from 2008 to 2011 in Tongling People＇s Hospital with pathologically confirmed lung adenocareinoma cancer tissues. Results In histopathology of lung adenocareinoma,EML4 - ALK fusion gene positive expression rate were 8.33%. The EGFR gene positive expression rate were 61.67%. The mutation rate of 76.92% （20/26）in the female patients was higher compared with male mutation rate of 50.0% （17/34） , and the P values was 0. 034. the mutation rate of patients with smoking 〈 400 years package of[ 74.29% （ 26/35 ） ] was higher compared with smoking≥400 mutation rate[ 44.0% （ 11/25 ） ], and the P values was 0. 017. the mutation rate in the ≥60 - year - old patients[42.86% （12/28） ]was lower compared with 〈60 - year - old mutation rate[78.13% （25/32） ] ,and the P val- ues was 0. 005. All the difference havd statistically significaee. The EMIA -ALK and EGFR of correlation analysis showed correlation（ R = - 0. 258,P = 0. 046 〈 0.05 ） ,and the difference was statistical. Conclusion EML4 - ALK positive expression was lower compared with EG- FR. The expression of EGFR and EML4 - ALK showed correlation and the difference had statistical significant.