HCV基因分型在丙肝诊断和治疗中的应用

Application of HCV Genotyping in the Diagnosis and Treatment of Hepatitis C

目的 建立ＨＣＶ基因分型方法，研究ＨＣＶ基因型与肝病程度的关系，探讨ＨＣＶ基因型与ＩＦＮ－ａ２ｂ抗病毒疗效的关系。方法 ＨＣＶ基因组ＮＳ５区ＰＣＲ扩增产物酶切分型。结果 ７３例ＨＣＶ感染者中有ＨＣＶⅡ型４５例，ＨＣＶⅢ型２８例。Ⅱ型ＨＣＶ感性肝炎３１例，肝硬化１４例；Ⅲ型ＨＣＶ感染者中有慢性肝炎２６例，肝硬化２例。Ⅱ型ＨＣＶ感染者的肝硬化发生率显著高于Ⅲ型ＨＣＶ感染者（Ｐ〈０．０５）。

Objective To establish technique for HCV genotyping and investigate the correlation between HCV genotypes and different severity of liver disease after HCV infection and the relationship between HCV genotypes and interferon therapy efficacy.Methods HCV genotypes were classified by non structural region 5 (NS5) of HCV genome polymerase chain reaction (PCR) product restriction endonuclease technique. Results The result of HCV genotyping suggested that 45 cases belong to type Ⅱ,28 type Ⅲ.In type Ⅱ of hepatitis C virus infection,31 patients had chronic hepatitis and 14 liver cirrhosis;In type Ⅲ of HCV infection,26 suffered from chronic hepatitis and 2 liver cirrhosis.The incidence rate of cirrhosis in HCV type Ⅱ patients was much highter than that in HCV type Ⅲ patients.All patients were treated with interferon alpha 2b (IFN α2b).Conclusion The incidence of liver cirrhosis was easier in type Ⅱ patients than in the type Ⅲ patients.When the patients suffered from HCV were treated with IFN α2b,the therapy efficacy in type Ⅲ was evidently better than that in type Ⅱ.