肿瘤相关基因对大肠肿瘤临床诊断及预后应用的研究

Tumor Related Genes on Clinical Diagnosis and Prognosis Application of Large Intestine Tumors

目的 探讨Ｒａｓ，Ｐ５３基因对大肠肿瘤的早期诊断及预后的临床应用价值。方法 用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）ＥＢ染色技术对２５例大肠肿瘤原发灶组织进行了Ｋｉ－，Ｎ－ｒａｓ第１处显子和Ｐ５３基因５～８外显子的点突变进行检测。息肉性腺瘤组织中１／２（５０％）发生了Ｋｉ－ｒａｓ点突变。在大肠癌组织中，存在Ｎ－ｒａｓ点突变、Ｋｉ－ｒａｓ点突变、Ｐ５３基因５～８外显子突变。

Objective To investigate Ras,P53 genes on clinical diagnosis and prognosis of large intestine tumors.Methods Using a polymerase chain reaction simple strand conformation polymorphism (PCR SSCP) EB staining technique,25 human coloretal tumors for mutation in exons 5,6,7,8 of P53 genes and exon 1 of Ki ,N ras were checked.Results Two polypoid adenoma cases were found Ki ras point mutation positive.In coloretal cancer teste,there were N ras mutation,Ki ras point mutation,and P53 genes mutation positive.Conclusion Ki ras point mutation existed in each stage early diagnosis in coloretal tumors.P53 exons point mutation rate was associated with metastasis of large intestine tumor.