PAX2和P53在卵巢浆液性肿瘤中的表达及其意义

Expression of PAX2 and P53 in ovarian serous tumors and its significance

目的:探讨PAX2和P53在卵巢浆液性肿瘤中的表达及其意义。方法:收集35例卵巢浆液性囊腺瘤、33例卵巢交界性浆液性囊腺瘤及50例卵巢浆液性囊腺癌标本,制作组织芯片;选取正常卵巢10例、包涵囊肿10例及输卵管内膜异位腺体或囊肿10例,采用免疫组织化学法检测PAX2、P53和Ki-67在其中的表达情况,分析其差异及临床病理意义。结果:①PAX2在正常卵巢表面上皮及包涵囊肿上皮中不表达,而在输卵管内膜异位腺体或囊肿上皮中有表达。②PAX2在卵巢浆液性囊腺瘤、交界性浆液性囊腺瘤及卵巢浆液性囊腺癌中的阳性率分别为86%(30/35)、67%(22/33)和16%(8/50),差异有统计学意义(P<0.01)。③P53在卵巢浆液性囊腺瘤、交界性浆液性囊腺瘤及卵巢浆液性囊腺癌中的阳性率分别为0%(0/35)、39%(13/33)和80%(40/50),差异有统计学意义(P<0.01)。④PAX2和P53的表达与卵巢癌患者的临床病理指标之间差异均无统计学意义。结论:①PAX2可能与卵巢浆液性肿瘤的良恶性分化有关;P53可能参与了卵巢癌的形成。②卵巢浆液性囊腺瘤、交界性浆液性囊腺瘤及低级别浆液性癌可能均来源于输卵管内膜异位腺体或囊肿;高级别浆液性癌的来源可能与之不同。

Objective： To explore and discuss the expression of PAX2 and P53 in ovarian serous tumors and their significance. Methods： Thirty five cases of ovarian serous cystadenoma, 33 cases of ovarian borderline serous cystadenoma, 50 cases of ovarian serous carcinoma, 10 cases of normal ovaries, 10 cases of inclusion cysts and 10 cases of endosalpingiosis gland or cyst were collected and made into tissue microarray. The expression of PAX2,P53 and Ki-67 was detected by immunohistochemistry. Their clinicopathological correlations were analyzed. Results： ①The expression of PAX2 in surface epithelium of normal ovarian and the epithelium of inclusion cyst were negative, whereas that in those of endosalpingiosis gland or cyst was positive. ②The positive rates of PAX2 in ovarian serous cystadenoma, ovarian borderline serous cystadenoma and ovarian serous carcinoma were 86% （30/35）, 67% （22/33） and 16% （8/50）, respectively. They are significantly different from each other （P〈0.01）. ③ The positive rates of P53 in ovarian serous cystadenoma, ovarian borderline serous cystadenoma and ovarian serous carcinoma were 0% （0/35）, 39% （13/33） and 80% （40/50） respectively. They are significantly different from each other （P〈0.01）.④The expression of PAX2 and P53 did not show significant correlation with patients＇ clinicopathological parameters. Conclusion： ① The expression of PAX2 may relate with the differentiation of ovarian benign and malignant tumors, P53 may be involved in the formation of ovarian cancer. ②Ovarian serous cystadenoma, ovarian borderline serous cystadenoma and low-grade ovarian serous carcinoma may originate from endosalpingiosis gland or cyst, but high-grade ovarian serous carcinoma may have a different origin.