高压氧对荷人喉咽鳞癌小鼠肿瘤生长的影响

Effects of hyperbaric oxygen on the tumor growth of human pharyngeal squamous cancer in mouse

目的探讨高压氧对荷人喉咽鳞癌小鼠肿瘤生长的影响。方法将32只雄性Balb／c小鼠按数字表法随机分为4组：A组（成瘤5d后处死）、B组（成瘤10d后处死）、C组（成瘤后高压氧暴露5d后处死）、D组（成瘤后高压氧暴露10d后处死），每组8只。接种经消化的对数期人喉咽鳞癌Fadu细胞，小鼠荷瘤后分别暴露于常压空气和高压氧中，各组相应处理后检测其肿瘤质量、体积，进行组织病理学及免疫组织化学分析。结果各组小鼠移植瘤组织病理学结果均为低分化鳞状细胞癌。C组肿瘤质量抑制率为20．13％、体积抑制率为20．54％，成纤维细胞活化蛋白叫FAPα）表达强度明显低于A组（P〈0．05）；D组肿瘤质量抑制率为13．65％、体积抑制率为10．30％，FAPα表达强度明显低于B组（P〈0．05）。结论高压氧对荷人喉咽癌小鼠肿瘤的生长有一定的抑制作用。

Objective To explore the effect of hyperbaric oxygen （HBO） on the tumor growth of human pharyngeal cancer in mouse model. Methods Thirty-two male Balb/e mice were randomly divided into 4 groups： group A or the control group （sacrificed 5 days after tumor loading） , group B （sacrificed 10 days after tumor loading ）, group C （sacrificed 5 days after tumor loading and HBO exposure ）, and group D （sacrificed 10 days after tumor loading and HBO exposure ）, each consisting of 8 animals. Following vaccination with the digested pharyngeal cancer Fadu cells in logarithmic phase, the tumor-loaded mice were respectively exposed to atmospheric air and HBO. Then, tumor volume, weight, histopathology and immunohistochemistry were detected for the animals of each group. Results Histopathology of the tumor-loaded mice in each group displayed low-differentiated squamous cell carcinoma. For the animals in group C, tumor mass inhibition rate was 20.13% , and volume inhibition rate was 20.54%. The expression level of fibroblast activation protein-alpha （FAPα） was obviously inferior to that of group A （ P 〈 0.05 ）. For the animals in group D, tumor mass inhibition rate was 13, 65% , and volume inhibition rate was 10.30% and the expression level of FAPα was obviously inferior to that of group B （ P 〈 0.05 ）. Conclusions HBO seemed to have certain effect on the inhibition of tumor growth of human pharyngeal cancer in mice.