荧光光谱法研究氯普噻吨的氧化反应及其分析应用

Studies on the Oxidation Reaction of the Chlorprothixene by the Spectrofluorimetry and Analytical Application

氯普噻吨本身不具有荧光 ,但氧化后可形成具有强荧光的产物 .本文系统地研究了以稀硝酸为氧化剂对氯普噻吨的氧化作用 ,提出了高灵敏度测量氯普噻吨的荧光光度分析新方法 .结果表明 :荧光强度与氯普噻吨的浓度在 0 .0 0 4～ 0 .1m g/ L范围内呈良好的线性关系 .该法简便 ,快速 ,灵敏度高 ,检出限可达 0 .0 0 2 mg/ L(6 .33× 10 - 9mol/ L) ,利用该法测量片剂中氯普噻吨的含量 ,结果令人满意 ,平均回收率为 99.6 % .

Chlorprothixene, a nofluorescent substance, can be transformed into a highly fluorescent product by oxidation. This paper studied the oxidation of chlorprothixene by reaction with the dilute nitric acid and developed a new high sensitive fluorimetric method with the detection limit of 0.002 mg/L (6.33×10 -9 mol/L). The linear relationship between the fluorescence intensity and the concentration of chlorprothixene is over the range of 0.004~0.1 mg/L. The method is simple, rapid and sensitive, and can be applied to the determination of chlorprothixene in the tablets with the satisfied result.