氯霉素前体和甲砜霉素前体的毛细管电泳手性分离

Chiral Resolution of Precursor of Chloramphenicol and Thiamphenicol by High Performance Capillary Electrophoresis

采用高效毛细管电泳 ,以羧甲基 -β-环糊精、羟丙基 -β-环糊精、二甲基 -β-环糊精作为手性添加剂对氯霉素前体和甲砜霉素前体对映体进行了分离 .并研究了手性选择剂的类型和浓度、背景电解质的酸度、背景电解质的类型、操作电压和温度等因素对对映体分离的影响 .分离结果表明 ,不仅为此类药物提供了简便、快速的分离分析方法 ,而且还获得了比以往报道更高的分离度 .

The methods were developed to separate enantimer of precursor of chloramphenicol ((±)\|threo\|1\|p\|nitrophenyl\|2\|amino\|1,3\|propanediol, NAPD) and enantiomer of precursor of thiamphenicol ((±)\|threo\|1\|p\|methylsulfonyl serine ethylester, MSSE). The optical isomers were resolved by High Performance Capillary Electrophoresis(HPCE) using carboxylmethyl\|β\|cyclodextrin(CM\|β\|CD)、hydroxypropyl\|β\|cyclodextrin(HP\|β\|CD) 、dimethyl\|β\|cyclodextrin(DM\|β\|CD) as chiral selectors. The separations were performed on a φ75 μm×50 cm uncoated capillary. The effects of the types and concentrations of CDs in buffer on chiral resolution were investigated. The effects of pH and the types of the background electrolytes on chiral resolution were also investigated and optimized. It was found that the NAPD enantiomer can be baseline separated by CM\|β\|CD、HP\|β\|CD and DM\|β\|CD. However, the MSSE enantiomer can only be baseline separated by CM\|β\|CD, partially separated by HP\|β\|CD and can not be chirally separated by DM\|β\|CD.