新生大鼠缺氧缺血性脑病SCN8A基因表达及病理学改变

Experimental study on hypoxic ischemic encephalopathy with expression of SCN8A gene and correlating with pathology

目的探讨新生大鼠缺氧缺血性脑病(HIE)SCN8A基因表达的变化规律及其与病理学改变的关系。方法采用Rice法制作新生大鼠HIE动物模型,于不同时间段处死大鼠,提取脑组织。HE染色观察脑组织病理学变化,RT-PCR方法检测脑组织中SCN8A基因的表达情况。结果缺氧缺血时间越长,脑细胞损伤愈明显。SCN8A基因表达水平在缺氧缺血3、7 d均升高(P<0.05)。结论缺氧缺血后脑组织SCN8A基因表达与病理学的变化一致,SCN8A基因在HIE的发生过程中发挥重要作用。

Objective To investigate the alteration tendency regularity of SCN8A gene expression and the relationship between the findings of SCN8A gene expression and those of pathology in the brains of neonatal rats models with hypoxic ischemic encephalopathy（HIE）.Methods HIE model was made by rice protocol.After that,the rats were executed to obtain brain tissue in different time respectively.HE dyeing was adopted to detect the results of brain tissue,the expression of mRNA of SCN8A was tested by RT-PCR.Results The hypoxic-ischemic damage from the TEM results showed a clear positive correlation of brain damage with exposure time,the brain cell was becoming demolished.Compared with that of control group,the level of SCN8A mRNA of 3days and 7days group was increased（P0.05）.Conclusions The changes of SCN8A gene expression are unanimously correlated to the pathological findings in HIE.SCN8A gene plays an important role in the process of hypoxic ischemic encephalopathy.