无针喷射控释双生长因子藻酸盐水凝胶对心肌梗死兔左室重塑及心功能的影响

Effect of needleless jetting two growth factors controlled-release alginate hydrogel on cardiac remodeling and function after myocardial infarction

目的探讨无针喷射控释双生长因子藻酸盐水凝胶改善心肌梗死兔左室重塑和心功能的可行性。方法成功建立40只兔心肌梗死模型后,随机分成5组:盐水组、凝胶组、VEGF-A165凝胶组、PDGF-BB凝胶组和双生长因子凝胶组。实验干预8 w后,测量平均瘢痕厚度进行形态学分析,采用超声心动图评价心功能,并进行免疫组化检测CD34及α-SMA的表达。结果凝胶组、VEGF-A165凝胶组、PDGF-BB凝胶组及双生长因子凝胶组的平均瘢痕厚度较盐水组明显增厚(P<0.05)。双生长因子凝胶组心功能较其他各组心功能有所改善(P<0.05)。VEGF-A165凝胶组、PDGF-BB凝胶组及双生长因子凝胶组的微血管及小动脉密度均有所增加,双生长因子凝胶组的血管密度增加最为明显(P<0.05)。结论无针喷射控释双生长因子藻酸盐水凝胶能够改善心肌梗死左室重塑及心功能,并增加心肌血管密度,为缺血性心脏病治疗提供一种新方法。

Objective To investigate whether needleless jetting two growth factors controlled-release alginate hydrogel can improve left ventricular remodeling and cardiac function after myocardial infarction.Methods After myocardial infarction models were prepared,40 rabbits were randomly assigned into five groups： saline,gel,VEGF-A165 gel,PDGF-BB gel and two growth factors gel groups.After procedure 8 weeks,morphological analysis was processed by measuring average scar thickness.Cardiac function was evaluated by echocardiography.The expressions of CD34 and α-SMA were detected using immunohistochemistry（IHC）.Results Results of morphological analysis showed that the average scar thickness of gel,VEGF-A165 gel,PDGF-BB gel and two growth factors gel groups was significantly thicker than the average scar thickness of saline group（P0.05）.The cardiac function was improved significantly in two growth factors gel group（P0.05）.The results of immunohistochemistry showed density of microvessels and small arteries were increased in VEGF-A165 gel,PDGF-BB gel and two growth factors gel groups,while the effect of two growth factors gel group was the most（P0.05）.Conclusions Needleless jetting two growth factors controlled-release alginate hydrogel can improve left ventricular remodeling and cardiac function after myocardial infarction and increase the density of myocardial vessels,which may provide a new strategy for the treatment of ischemic heart disease.