转入OsLTP对甘蓝型油菜耐盐水平的影响

Effects of OsLTP Gene on Salt Tolerance of Transgenic Brassica napus

【目的】验证OsLTP对油菜耐盐胁迫的影响。【方法】构建高效表达载体pCAM2300-35S-LTP-Ocs,使用农杆菌介导的油菜下胚轴转化方法,将来自巴西旱稻的OsLTP导入到甘蓝型油菜扬油6号中。通过PCR和Southern杂交验证转基因植株,并测定不同浓度NaCl胁迫下转OsLTP植株的生物量和生理指标,鉴定其耐盐性。【结果】分子鉴定表明运用上述方法成功地将外源OsLTP导入甘蓝型油菜中。在100 mmol.L-1NaCl和200 mmol.L-1NaCl处理下,转基因后代植株的生物量、叶绿素积累量、PSⅡ活性和叶片抗氧化酶活性均比非转基因植株高,而叶片MDA含量低于对照。【结论】导入的OsLTP通过保持叶片中PSⅡ的活性,维持叶绿素的积累,提高抗氧化酶活性等途径来提高转基因甘蓝型油菜的耐盐水平。

[ Objective ] This study aimed to understand the function of OsLTP gene under salt stress. [ Method ] An efficient expression vector of pCAM2300-35S-LTP-Ocs harboring OsLTP gene from Brazilian upland rice was transferred into Brassica napuscv.Yangyou 6 viaAgrobacterium-mediatedgenetic transformation of therapeseedhypocotyls. [ Result ] The integration of the foreign OsLTP gene was confirmed by PCR and southern hybridization analysis. In the treatments of 100 mmol·L-1 and 200 mmol.Lz NaC1 solution, the biomass of plants, chlorophyll accumulation, PS II activity, and antioxidant enzyme activities in leaves of the transgenic plants were higher than that of the non-transformants, MDA content in leaves was insteadly lower than the controls. [Conclusion] OsLTP gene improved salt tolerance of transgenic B. napus by maintaining PSII activity, chlorophyll accumulation in leaves and increased the activities of antioxidant enzymes.