高浓度葡萄糖通过下调自噬增加体外培养雪旺细胞的凋亡

High concentration of glucose increases apoptosis of Schwann cell in vitro by downregulation of autophagy

目的观察高浓度葡萄糖对雪旺细胞自噬活性的影响以及自噬对高糖条件下雪旺细胞增殖活性及凋亡的影响。方法体外培养RSC96细胞,Western blot和免疫荧光法检测beclin1和caspase-3蛋白表达,MTT检测细胞增殖活性。结果高浓度葡萄糖组RSC96细胞beclin1的表达减少(P<0.05),细胞增殖活性降低(P<0.05,P<0.01)。自噬被抑制后加剧了高糖对细胞增殖活性的削弱作用(P<0.01),同时增加了高糖促进caspase-3的活化作用(P<0.01)。结论高浓度葡萄糖通过下调自噬活性增加细胞凋亡。

Objective To observe the effect of the high concentration of glucose on autophagy of rat＇s Schwann cells in vitro and the effect of autophagy on the proliferation and apoptosis of Sehwann cells cultured in high glucose me- dium. Methods RSC96 cells were cultured. The expression of beclinl and caspase-3 were detected by immunoflu- orescence and Western blot. The proliferation was detected by MTT. Results The expression of beclinl in RSC96 cells cultured in high glucose medium was reduced （ P 〈 0. 05 ） , as well as the proliferation was decreased （ P 〈 0. 05, P 〈0.01 ）. Inhibition of autophagy resulted in aggravating the decrease of the proliferation of RSC96 cells cultured in high glucose medium （P 〈 0.01 ） and upregulating the activity of caspase-3 （P 〈0. 01 ）. Conclusions The impairment of the high concentration of glucose increased apoptosis by downregulation of autophagy.