儿童与成人弥漫大B细胞淋巴瘤的Hans、Choi免疫分型和C-MYC基因特征

Hans,Choi immunophenotyping and C-MYC genetic features between pediatric and adult diffuse large B-cell lymphoma

目的探讨弥漫大B细胞淋巴瘤(DLBCL)各年龄组的Hans、Choi免疫分型及C-MYC基因特征。方法收集60例DLBCL临床病理资料并进行随访,其中儿童组17例、成人组43例。用免疫组化SP法观察CD10、BCL-6、MUM1、FOXP1、GCET1及CD5蛋白表达,根据Hans及Choi免疫分型标准分型;用间期荧光原位杂交法检测C-MYC基因。结果 1)Hans分型:儿童DLBCL组GCB型11例,non-GCB型6例;成人组DLBCL中GCB型9例,non-GCB型34例(P<0.05)。2)Choi分型:儿童DLBCL组GCB型13例,ABC型4例;成人DLBCL组GCB型13例,ABC型30例(P<0.01)。3)C-MYC基因:儿童组DLBCL中C-MYC基因断裂6例;成人组DLBCL中C-MYC基因正常43例(P<0.01)。结论儿童DLBCL以GCB型为主,预后较好;成人DLBCL以non-GCB或ABC型为主,预后相对较差,儿童DLBCL的C-MYC基因断裂明显高于成人。

Objective To explore the difference of Hans, Choi immunophenotyping and C-MYC gene characteris- tics between pediatric and adult diffuse large B-cell lymphoma. Methods To collect 60 cases of DLBCL clinico- pathological data and follow-up ,which contained 17 cases of children group, adult group of 43 patients. To observe CD10, BCL-6, MUMI, FOXP1, GCET1 and CD5 protein expression by immunohistochemical SP method and eval- uated by Hans and Choi immunophenotyping standard genotyping. To detect C-MYC gene circumstances by inter- phase fluorescence insituhybridization （FISH）. Results 1 ） Hans typing： the Children DLBCL group of GCB were 11 cases, non-GCB type were 6 cases; the GCB type in the adult DLBCL group were nine cases, non-GCB type were 34 cases. 2）Choi typing：the Children DLBCL group of GCB type were 13 cases, ABC type were 4 eases; the adult DLBCL group of GCB type were 13 cases, ABC type were 30 cases （P 〈0. 01 ）. 3） C-MYC gene：The C-MYC gene disruption of children DLBCL were 5 cases; C-MYC gene in 43 adult cases were normal（P 〈0. 01 ）. Conclu- sions Child DLBCL of GCB-type with better prognosis while but non-GCB-type predict worse prognosis. The C-MYC gene disruption in Children DLBCL was significantly higher than that of adult ones.