DNAzyme在金电极上自组装电化学生物传感器的制备

The Preparation of Electrochemical Biosensor with Self-assembled DNAzyme on Gold Electrode

利用巯基与金表面Au-S键的自组装技术,可以将巯基修饰的DNAzyme固定在金电极表面形成电化学生物传感器,并以含有[Fe(CN)6]3-/4-的磷酸缓冲液(PBS)作为电化学检测底液,利用循环伏安法初步研究该修饰电极的电化学行为和修饰过程的电化学表征.DNAzymes自组装膜的存在明显抑制了二茂铁与[Fe(CN)6]3-/4-之间的电子转移.结果表明,基于巯基修饰的DNAzyme在金电极表面能够发生自组装作用,在金电极表面形成较为稳固的自组装DNAzyme膜.

This paper reports the electrochemical biosensor using DNAzyme with thiolated- modified through Au-S self-assembly technology, which is bound on the surface of the gold electrode, and utilize phosphate buffer solution （PBS） with [ Fe（CN） 6 ] 3 -/4- as the solu- tion for the electrochemical detection. Then, we study the electrochemical characterization and modification process of the modified electrode by cyclic vohammetry, the electrochemi- cal transfer significantly decreased between [ Fe（CN） 6 ] 3-/4- and Fc on the gold electrode modified with DNAzymes. The experimental results show that the surface of gold electrode can self-assemble based on thiolated-modified DNAzyme, and stable self-assembled DNAzyme can form on the surface of gold electrode.