NUMB表达对肝细胞癌患者预后的影响

Influence of NUMB Expression on Prognosis in Patients with Hepatocellular Carcinoma

目的探讨NUMB在肝细胞癌(肝癌)中的表达及其与患者临床因素、预后的关系。方法根治性手术切除的肝癌病理标本119例,使用免疫组织化学(IHC)方法检测肝癌组织及癌旁组织中NUMB蛋白,并分析NUMB的表达与患者临床因素及预后的关系。结果肝癌组织中NUMB表达阳性率为36.1%(43/119),明显低于癌旁组织的100.0%(119/119)(P<0.01);NUMB表达缺失与肿瘤分化程度、脉管侵犯、早期复发及BCLC分期相关(P<0.05);NUMB表达缺失的患者,总体生存率及无瘤生存率均下降(P<0.01)。Cox多因素分析显示NUMB为影响肝癌患者预后的独立危险因素(P<0.01)。结论 NUMB在肝癌组织中表达缺失,并且其表达的缺失与肝癌侵袭特性有关,为影响患者预后的独立危险因素。

Objective To investigate NUMB expression in hepatocellular carcinoma（HCC） patients as well as its correlation with HCC patients’clinicopathological characteristics and prognosis.Methods Clinical samples were obtained from 119 HCC patients who had undergone curative liver resection.Immunohistochemistry assay was used to detect NUMB protein in HCC tissues and adjacent non-tumor tissues.The correlation of NUMB expression with clinicopathological characteristics and prognosis was analyzed.Results The positive rate of NUMB expression in HCC tissues was 36.1%（43 /119）,which was significantly lower than that in adjacent tissues [100%（119 /119）,P〈0.01];Deficiency of NUMB expression was associated with worse tumor differentiation,vascular invasion,early recurrence and advanced BCLC stage（P〈0.05）;HCC patients with deficiency of NUMB expression had shorter overall survival and recurrence-free survival（P〈0.01）.Cox multivariate analyses showed that NUMB was an independent risk factor of HCC patients’prognosis（P〈0.01）.Conclusion NUMB expression is markedly down-regulated in HCC tissues.Deficiency of NUMB expression correlates with the characteristics of invasion in HCC.NUMB is an independent risk factor of prognosis in HCC.