猴腿蹄盖蕨总多酚的超声提取工艺研究

Study on purification technology of total polyphenols from Athyrium Mult identatum (Doll.) ching. with ultrasonic extraction method

以总多酚提取率为主要考察指标，探索了猴腿蹄盖蕨总多酚的超声提取工艺条件。通过单因素和正交试验确定了猴腿蹄盖蕨总多酚的最佳超声提取工艺条件为料液比为1：30、乙醇浓度为65％、超声提取3次、每次45min，提取温度为75—80℃。在上述超声提取条件下．猴腿蹄盖蕨总多酚的提取率可以达到169．27mg／g，上述提取工艺条件可用于猴腿蹄盖蕨总多酚的超声提取。

To explore the optimum extraction technology of total polyphenols （TP） in A thyrium Mult identatum （Doll.） Ching.with ultrasonic extraction method, the extraction efficiency of TP was used as evaluating indicators. The optimum extraction conditions were obtained according to the single factor and orthogonal test adsorption and desorption rate as evaluating indicators. The ratio between raw material and solvent, the concentration of ethanol, the extraction times, and the extraction temperaturetime were 1：30, 65%, 3 times and 45 min respectively at 75-80℃. The extraction efficiency of TP in Athyrium Mult identatum （Doll.） Ching. can reach to 169.27 mg/g . under Under the optimum extraction conditions conditions which, it indicates that the extraction conditions obtained can be used for extracting TP in Athyrium Mult identatum（Doll.） Ching. with ultrasonic method