薄层色谱扫描法测定补气生血贴中阿魏酸的皮肤透过率

TLCS Determination of ferulic acid skin permeation in qi and blood paste

目的:建立薄层色谱扫描法(TLCS)测定补气生血贴中阿魏酸的皮肤透过率的方法。方法:分别选取3位受试者A、B、C,将贴片贴于神厥穴,贴药时间分别为12h、24h、36h,然后采用薄层色谱扫描法分别测定贴前与贴后不同时间阿魏酸的含量。层析条件:日本岛津CS-930反射线性扫描,λR=370nm,λs=310nm,SX=3。结果:阿魏酸在0.6～2.6μg内呈良好的线性关系。阿魏酸含量随着贴片时间的增长而减少,表明阿魏酸的皮肤透过率随贴时间的增大而增大。结论:实验表明贴片的有效成分阿魏酸可透过皮肤被吸收,并且吸收良好,为复方中药贴片的质量控制指标提供了依据。

Objective Establish the method to determine the ferulic acid skin permeation in qi and blood paste by TLCS.Method Three subjects A、B、C were selected,the pasters were sticked on shenjue acupoints for 12h、24h、36h separately.Then ferulic acid contents were determined at different time before and after the paste by TLCS.Chromatographic conditions： Japanese Shimadzu CS 930 reflective linear scanning,λR = 370nm,λs = 310nm,SX = 3.Result Ferulic acid showed a good linear relationship in 0.6 2.6μg.Ferulic acid content decreased with the growth of time,showing that ferulic acid skin permeation increased as the paste time growed.Conclusion The experiment demonstrates that the effective constituent Ferulic acid could be well absorbed through the skin.It provides a basis for the quality control indicator of compound traditional Chinese medicine patch.