高效液相色谱法测定灵芝提取物中尿苷和腺苷含量

HPLC Determination of Uridine and Adenosine Contents in Ganoderma Extracts

目的建立测定不同产地的灵芝提取物(包括赤芝提取物和紫芝提取物)中尿苷和腺苷含量的反相高效液相色谱法。方法采用Agilent Zorbax SB-Aq柱(250 mm×4.6 mm,5μm),以乙腈-水为流动柱(梯度洗脱),流速为0.8 mL/min,检测波长为260 nm。结果尿苷和腺苷质量浓度分别在2.44～195.6μg/mL(r=1.000 0)和2.64～105.6μg/mL(r=1.000 0)范围内与峰面积呈良好的线性关系,平均回收率分别为102.0%和101.2%,RSD分别为0.2%和1.0%(n=6)。不同产地的灵芝提取物中尿苷及腺苷的含量及二者总量差异较大,且同一产地不同批次尿苷及腺苷的含量及二者总量也存在一定差异。结论该法简便、准确、重复性好,可用于灵芝提取物中尿苷和腺苷的测定,试验结果为灵芝提取物的质量评价提供了参考依据。

Objective To establish an RP-HPLC method for the simultaneous determination of uridine and adenosine contents in Ganoderma extracts from different habitats.Methods The Agilent Zorbax SB-Aq column（ 250 mm × 4.6 mm,5 μm） was adopted with the mobile phase of acetonitrile-water by the gradient elution.The flow rate was 1.0 mL / min.The detection wavelength was set at 260 nm.Results The linear ranges of uridine and adenosine were（ 2.44-195.6） μg /mL（ r = 1.000 0） and（ 2.64-105.6） μg /mL（ r = 1.000 0）.The average recovery rates were 102.0% and 101.2%,RSD was 0.2% and 1.0%（ n = 6） respectively.The uridine and adenosine contents of samples had significant differences in different habitats,moreover the contents of uridine and adenosine were also different in different batches from the same habitat.Conclusion This method is simple,accurate,repeatable,which can be used to determine the contents of uridine and adenosine in Ganoderma extracts and provide the referenc basis for the quality assessment of Ganoderma extracts.