手足口病重症患者的临床表现

Clinical manifestation of cases with severe hand-foot and mouth disease

目的探索重症手足口病患者中肠道病毒EV71基因特征。方法对采集的重症手足口患者标本进行实时荧光定量PCR检测后,阳性标本进行病毒分离培养,选取分离毒株,对其VP1区进行核苷酸序列测定并构建系统发生树,且进行核苷酸和氨基酸的同源性比较。结果 106例手足口重症患者,实时荧光定量检测结果 47例阳性,47例核酸检测阳性标本病毒分离到19株毒株,对15株毒株VP1进行测序后,确定均为C4a亚型,核苷酸和氨基酸同源性比较分别为98.3%～99.7%,99.3%～100%。结论 2010-2011年银川市手足口重症病例EV71株均为C4a亚型,与2008年宁夏报道的流行亚型相一致,提示C4a亚型是近年来宁夏手足口病流行的主要亚型。

Objective To explore the genetic characteristics of severe hand-foot-mouth disease by enterovirus EV71.Methods The specimens of patients with severe hand-foot-mouth disease were collected after the detection of the real-time PCR positive specimens for virus isolation,selected the isolates,their VP1 region were sequenced and phylogenetic tree,and its nucleotide and amino acid homology.Results In 106 cases with severe hand-foot-mouth disease the real-time fluorescence quantitative test of 47 cases were positive,47 cases of nucleic acid testing positive specimens of the virus isolated 19 strains,15 strains VP1 sequencing.It was determined as C4a subtype,and comparison of nuclear the nucleotide and amino acid homology was 98.3% ～ 99.7% and 99.3% ～ 100%.Conclusion EV71 strains are C4a subtypes in patients with severe hand-foot-mouth disease in Yinchuan between 2010 and 2011,it is similar to that in Ningxia in 2008,the C4 subtypes epidemic in Ningxia is main subtypes of foot and mouth disease recent years.