褐藻胶抗铅污染对雄性小鼠生殖的影响

Effects of alginate on reproductive function in male mice with lead poisoning

目的通过建立铅中毒模型，研究褐藻胶对醋酸铅染毒雄性小鼠生殖的影响。方法将雄性昆明种小鼠随机分为空白组、模型组、青霉胺组、褐藻胶低、中、高剂量组。除空白组外，其他组腹腔注射醋酸铅，以相应药物治疗。30d后与正常雌鼠交配两个性周期，交配结束后处死雄性小鼠测定血铅水平、雄性器官指数、精子畸形率活动度。在同笼后的15～21d处死雌鼠，记录怀孕鼠数及孕鼠平均胎数。结果模型组雄鼠的血铅浓度高于空白组及各实验组（P〈0．05）；褐藻胶组雄鼠的睾丸、附睾脏器指数高于模型组（P〈0．05），精子畸形率低于模型组（P〈0．05），活动率高于模型组（P〈0．05），与褐藻胶组雄性小鼠交配的怀孕雌鼠数及孕鼠平均胎数均高于模型组（P〈0．01）。结论褐藻胶对醋酸铅染毒雄性小鼠生殖有保护作用。

Objective To study the effects of alginate on the reproductive system of male mice with lead poisoning. Methods The mice were randomly divided into the blank group, the model group, the penicillamine group, and three alginate groups. Mice in the other five groups were intraperitoneally injected with plumbic acetas except mice in the blank group, and then mice were treated with the corresponding medicine in the penicillamine group, and three alginate groups. Subsequently mice were mated with sexually mature females and their reproductive ability was evaluated. Results （1） Level of blood lead in the model group was higher than that of the control group （P〈0.05）; （2）The testis index and epididymis index in alginate groups were higher than that of the model group （P〈0.05）; （3）The rate of sperm deformity in alginate group was lower than that of the model group （P〈0.05）, while the activity rate was higher than that of the model group （P〈0.05）; （4）The rates of pregnancy in alginate groups were higher than those the model group （P〈0.05）. Conclusion Alginate had protective effects on the reproductive system of mice with lead poisoning.